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Literature summary for 3.2.1.78 extracted from
- Molecular cloning and characterization of a novel cold-active beta-1,4-D-mannanase from the Antarctic springtail, Cryptopygus antarcticus (2008), Comp. Biochem. Physiol. B, 151, 32-40.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| exprression in Escherichia coli fused with thioredoxin gene | Cryptopygus antarcticus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 5 mM, 24.8% residual activity | Cryptopygus antarcticus |  |
| Hg2+ | 5 mM, 16.4% residual activity | Cryptopygus antarcticus | |
| Mn2+ | 5 mM, 32% residual activity | Cryptopygus antarcticus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 5 mM, 228% of initial activity | Cryptopygus antarcticus | |
| Cu2+ | 5 mM, 151% of initial activity | Cryptopygus antarcticus | |
| Mg2+ | 5 mM, 178% of initial activity | Cryptopygus antarcticus | |
| Zn2+ | 5 mM, 220% of initial activity | Cryptopygus antarcticus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cryptopygus antarcticus | B4XC07 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 416.3 | - |
30°C, pH 3.5 | Cryptopygus antarcticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| locust bean gum + H2O | - |
Cryptopygus antarcticus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Cryptopygus antarcticus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
5 | 20-40% of maximum activity | Cryptopygus antarcticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
half-life less than 10 min | Cryptopygus antarcticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
- |
Cryptopygus antarcticus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | 6 | more than 50% of maximum activity | Cryptopygus antarcticus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
inactivation above | Cryptopygus antarcticus |
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Release 2023.1 (January 2023)
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