Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Vibrio sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, more than 80% inhibition | Vibrio sp. | |
Al3+ | 1 mM, more than 80% inhibition | Vibrio sp. | |
Cu2+ | 1 mM, more than 80% inhibition | Vibrio sp. | |
Hg2+ | 1 mM, more than 80% inhibition | Vibrio sp. | |
Pb2+ | 1 mM, more than 80% inhibition | Vibrio sp. | |
Zn2+ | 1 mM, more than 80% inhibition | Vibrio sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km value for konjac glucomannan 0.6 mg/ml | Vibrio sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
gel fitlration | Vibrio sp. |
73000 | - |
1 * 76309, calculated including signal peptide, 1 * 73588, calculated, mature protein, 1 * 73000, SDS-PAGE | Vibrio sp. |
73588 | - |
1 * 76309, calculated including signal peptide, 1 * 73588, calculated, mature protein, 1 * 73000, SDS-PAGE | Vibrio sp. |
76309 | - |
1 * 76309, calculated including signal peptide, 1 * 73588, calculated, mature protein, 1 * 73000, SDS-PAGE | Vibrio sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio sp. | B5BQ69 | - |
- |
Vibrio sp. MA-138 | B5BQ69 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | sequence contains a signal peptide of 26 amino acids | Vibrio sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glucomannan + H2O | from konjac flour | Vibrio sp. | ? | - |
? | |
glucomannan + H2O | from konjac flour | Vibrio sp. MA-138 | ? | - |
? | |
additional information | enzyme has the ability to bind soluble beta-mannans | Vibrio sp. | ? | - |
? | |
additional information | enzyme hydrolyzes homopolysaccharides such as codium mannan, Porphyra mannan, coffee mannan, and coconut mannan, to form mainly mannobiose and mannotriose in addition to minor sugars, such as mannose, mannotetraose, and mannopentaose | Vibrio sp. | ? | - |
? | |
additional information | enzyme has the ability to bind soluble beta-mannans | Vibrio sp. MA-138 | ? | - |
? | |
additional information | enzyme hydrolyzes homopolysaccharides such as codium mannan, Porphyra mannan, coffee mannan, and coconut mannan, to form mainly mannobiose and mannotriose in addition to minor sugars, such as mannose, mannotetraose, and mannopentaose | Vibrio sp. MA-138 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 76309, calculated including signal peptide, 1 * 73588, calculated, mature protein, 1 * 73000, SDS-PAGE | Vibrio sp. |
Synonyms | Comment | Organism |
---|---|---|
Man5C | - |
Vibrio sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Vibrio sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
stable up to, for at least 20 min | Vibrio sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Vibrio sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10 | more than 80% of original activity after 12 h incubation at 4°C | Vibrio sp. |