Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.78 extracted from

  • Tanaka, M.; Umemoto, Y.; Okamura, H.; Nakano, D.; Tamaru, Y.; Araki, T.
    Cloning and characterization of a beta-1,4-mannanase 5C possessing a family 27 carbohydrate-binding module from a marine bacterium, Vibrio sp. strain MA-138 (2009), Biosci. Biotechnol. Biochem., 73, 109-116.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Vibrio sp.

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, more than 80% inhibition Vibrio sp.
Al3+ 1 mM, more than 80% inhibition Vibrio sp.
Cu2+ 1 mM, more than 80% inhibition Vibrio sp.
Hg2+ 1 mM, more than 80% inhibition Vibrio sp.
Pb2+ 1 mM, more than 80% inhibition Vibrio sp.
Zn2+ 1 mM, more than 80% inhibition Vibrio sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km value for konjac glucomannan 0.6 mg/ml Vibrio sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
gel fitlration Vibrio sp.
73000
-
1 * 76309, calculated including signal peptide, 1 * 73588, calculated, mature protein, 1 * 73000, SDS-PAGE Vibrio sp.
73588
-
1 * 76309, calculated including signal peptide, 1 * 73588, calculated, mature protein, 1 * 73000, SDS-PAGE Vibrio sp.
76309
-
1 * 76309, calculated including signal peptide, 1 * 73588, calculated, mature protein, 1 * 73000, SDS-PAGE Vibrio sp.

Organism

Organism UniProt Comment Textmining
Vibrio sp. B5BQ69
-
-
Vibrio sp. MA-138 B5BQ69
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification sequence contains a signal peptide of 26 amino acids Vibrio sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glucomannan + H2O from konjac flour Vibrio sp. ?
-
?
glucomannan + H2O from konjac flour Vibrio sp. MA-138 ?
-
?
additional information enzyme has the ability to bind soluble beta-mannans Vibrio sp. ?
-
?
additional information enzyme hydrolyzes homopolysaccharides such as codium mannan, Porphyra mannan, coffee mannan, and coconut mannan, to form mainly mannobiose and mannotriose in addition to minor sugars, such as mannose, mannotetraose, and mannopentaose Vibrio sp. ?
-
?
additional information enzyme has the ability to bind soluble beta-mannans Vibrio sp. MA-138 ?
-
?
additional information enzyme hydrolyzes homopolysaccharides such as codium mannan, Porphyra mannan, coffee mannan, and coconut mannan, to form mainly mannobiose and mannotriose in addition to minor sugars, such as mannose, mannotetraose, and mannopentaose Vibrio sp. MA-138 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 76309, calculated including signal peptide, 1 * 73588, calculated, mature protein, 1 * 73000, SDS-PAGE Vibrio sp.

Synonyms

Synonyms Comment Organism
Man5C
-
Vibrio sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Vibrio sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
stable up to, for at least 20 min Vibrio sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Vibrio sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 10 more than 80% of original activity after 12 h incubation at 4°C Vibrio sp.