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Literature summary for 3.2.1.78 extracted from
- Cloning, functional expression and characterization of Aspergillus sulphureus beta-mannanase in Pichia pastoris (2007), J. Biotechnol., 128, 452-461.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Aspergillus sulphureus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km value for locust bean gum at pH 2.4, 50°C is 0.93 mg/ml | Aspergillus sulphureus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aspergillus sulphureus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
x * 41389, calculated, x * 48000, SDS-PAGE of glycosylated enzyme, x * 39000, SDS-PAGE of deglycosylated enzyme | Aspergillus sulphureus |
| 41389 | - |
x * 41389, calculated, x * 48000, SDS-PAGE of glycosylated enzyme, x * 39000, SDS-PAGE of deglycosylated enzyme | Aspergillus sulphureus |
| 48000 | - |
x * 41389, calculated, x * 48000, SDS-PAGE of glycosylated enzyme, x * 39000, SDS-PAGE of deglycosylated enzyme | Aspergillus sulphureus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus sulphureus | Q2LE69 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | treatment with Endo H leads to 30% loss of enzymic activity and a reduction in molecular weight by about 9 kDa | Aspergillus sulphureus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture fluid | - |
Aspergillus sulphureus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 366 | - |
pH 2.4, 50°C | Aspergillus sulphureus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| enzyme is stable at room temperature for at least 2 days | Aspergillus sulphureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| locust bean gum + H2O | - |
Aspergillus sulphureus | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 41389, calculated, x * 48000, SDS-PAGE of glycosylated enzyme, x * 39000, SDS-PAGE of deglycosylated enzyme | Aspergillus sulphureus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Aspergillus sulphureus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
stable below | Aspergillus sulphureus |
| 60 | - |
half-life 50 min | Aspergillus sulphureus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2.4 | - |
- |
Aspergillus sulphureus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.2 | 8 | - |
Aspergillus sulphureus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus sulphureus | isoelectric focusing | - |
4.9 |
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