Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Aspergillus sulphureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km value for locust bean gum at pH 2.4, 50°C is 0.93 mg/ml | Aspergillus sulphureus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Aspergillus sulphureus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
x * 41389, calculated, x * 48000, SDS-PAGE of glycosylated enzyme, x * 39000, SDS-PAGE of deglycosylated enzyme | Aspergillus sulphureus |
41389 | - |
x * 41389, calculated, x * 48000, SDS-PAGE of glycosylated enzyme, x * 39000, SDS-PAGE of deglycosylated enzyme | Aspergillus sulphureus |
48000 | - |
x * 41389, calculated, x * 48000, SDS-PAGE of glycosylated enzyme, x * 39000, SDS-PAGE of deglycosylated enzyme | Aspergillus sulphureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus sulphureus | Q2LE69 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | treatment with Endo H leads to 30% loss of enzymic activity and a reduction in molecular weight by about 9 kDa | Aspergillus sulphureus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture fluid | - |
Aspergillus sulphureus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
366 | - |
pH 2.4, 50°C | Aspergillus sulphureus |
Storage Stability | Organism |
---|---|
enzyme is stable at room temperature for at least 2 days | Aspergillus sulphureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
locust bean gum + H2O | - |
Aspergillus sulphureus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 41389, calculated, x * 48000, SDS-PAGE of glycosylated enzyme, x * 39000, SDS-PAGE of deglycosylated enzyme | Aspergillus sulphureus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Aspergillus sulphureus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable below | Aspergillus sulphureus |
60 | - |
half-life 50 min | Aspergillus sulphureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2.4 | - |
- |
Aspergillus sulphureus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.2 | 8 | - |
Aspergillus sulphureus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aspergillus sulphureus | isoelectric focusing | - |
4.9 |