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Literature summary for 3.2.1.78 extracted from

  • Marraccini, P.; Rogers, W.J.; Allard, C.; Andre, M.L.; Caillet, V.; Lacoste, N.; Lausanne, F.; Michaux, S.
    Molecular and biochemical characterization of endo-beta-mannanases from germinating coffee (Coffea arabica) grains (2001), Planta, 213, 296-308.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
both ManA and ManB Coffea arabica

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 43000, ManB, SDS-PAGE Coffea arabica

Organism

Organism UniProt Comment Textmining
Coffea arabica
-
 isozymes ManA, ManB with at least 7 isomers
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein two putative N-glycosilation sites, ManB Coffea arabica

Source Tissue

Source Tissue Comment Organism Textmining
seed germinating Coffea arabica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1400
-
 ManB, 37°C, pH 5.0 Coffea arabica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
mannohexaose + H2O
-
 Coffea arabica 2 mannotriose main product ?
mannopentaose + H2O
-
 Coffea arabica mannotriose + mannobiose main products ?
additional information no substrate: mannotriose, mannobiose, enzyme requires a minimum of four sugar units in substrates Coffea arabica ?
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, ManB, SDS-PAGE Coffea arabica

pI Value

Organism Comment pI Value Maximum pI Value
Coffea arabica chromatofocusing, 7 isomers
-
 5.5