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Literature summary for 3.2.1.78 extracted from

  • Akino, T.; Nakamura, N.; Horikoshi, K.
    Characterization of three beta-mannanases of an alkalophilic Bacillus sp. (1988), Agric. Biol. Chem., 52, 773-779.
No PubMed abstract available
Search for more literature for 3.2.1.78

Activating Compound

Activating Compound Comment Organism Structure
cysteine slight activation Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
 Bacillus sp. (in: Bacteria)
N-bromosuccinimide
-
 Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information copra beta-mannan: 2.0 mg/ml, locust bean beta-mannan: 3.8 mg/ml, konjak beta-mannan: 7.7 mg/ml, carob galactomannan: 0.9 mg/ml Bacillus sp. (in: Bacteria)

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 M-III, SDS-PAGE Bacillus sp. (in: Bacteria)
58000
-
 M-I, SDS-PAGE Bacillus sp. (in: Bacteria)
59000
-
 M-II, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
 M-I, M-II, M-III
-

Purification (Commentary)

Purification (Comment) Organism
M-I, M-II, M-III Bacillus sp. (in: Bacteria)

Source Tissue

Source Tissue Comment Organism Textmining
endosperm
-
 Bacillus sp. (in: Bacteria)
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bacillus sp. (in: Bacteria)
470
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
galactoglucomannan + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
glucomannan + H2O
-
 Bacillus sp. (in: Bacteria) oligosaccharides
-
 ?
mannan + H2O
-
 Bacillus sp. (in: Bacteria) oligosaccharides
-
 ?
mannopentaose + H2O
-
 Bacillus sp. (in: Bacteria) mannose + mannobiose + mannotriose + mannotetraose
-
 ?
mannotetraose + H2O
-
 Bacillus sp. (in: Bacteria) mannobiose + mannose + mannotriose small amounts of mannose and mannotriose ?
mannotriose + H2O
-
 Bacillus sp. (in: Bacteria) mannobiose + mannose
-
 ?
additional information not: mannobiose Bacillus sp. (in: Bacteria) ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
-
 Bacillus sp. (in: Bacteria)
60
-
 M-I, M-II Bacillus sp. (in: Bacteria)
65
-
 M-III Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
-
 Bacillus sp. (in: Bacteria)
50 70
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
-
 Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
-
 Bacillus sp. (in: Bacteria)
8.5
-
 M-III, at 65°C Bacillus sp. (in: Bacteria)
9
-
 M-I, M-II, at 60°C Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
-
 Bacillus sp. (in: Bacteria)
6 10
-
 Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
-
 Bacillus sp. (in: Bacteria)