Any feedback?
Literature summary for 3.2.1.77 extracted from
- Studies on the structure of yeast mannan. I. Purification and some properties of an alpha-mannosidase from an Arthrobacter species (1969), J. Biol. Chem., 244, 1043-1051.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Arthrobacter sp. |  |
| EDTA | - |
Arthrobacter sp. | |
| Mg2+ | - |
Arthrobacter sp. | |
| Zn2+ | - |
Arthrobacter sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
Mannan | based on molecular weight of 22000 from intact S. cerevisiae mannan | Arthrobacter sp. | |
| 0.5 | 2 | oligosaccharides from mannan | molecular weight of mannan multiplied by factor 50 describes molar concentration of side chains in the mannan molecule | Arthrobacter sp. |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Arthrobacter sp. | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | restores activity after EDTA treatment | Arthrobacter sp. | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| mannan + H2O | Arthrobacter sp. | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arthrobacter sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially | Arthrobacter sp. |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -10°C, indefinitely, | Arthrobacter sp. |
| 20°C, one drop of toluene, 1 day, 15% decline in activity | Arthrobacter sp. |
| 4°C, 2 days, | Arthrobacter sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| mannan + H2O | - |
Arthrobacter sp. | mannose + 1,6-alpha-D-mannan-backbone | further slow degradation | ? | |
| mannan + H2O | - |
Arthrobacter sp. | ? | - |
? | |
| oligosaccharides from mannan + H2O | - |
Arthrobacter sp. | mannose + ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
not stable above | Arthrobacter sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7 | mannan | Arthrobacter sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | mannan | Arthrobacter sp. |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
