Literature summary for 3.2.1.76 extracted from

Cardon, F.; Pallisse, R.; Bardor, M.; Caron, A.; Vanier, J.; Ele Ekouna, J.P.; Lerouge, P.; Boitel-Conti, M.; Guillet, M.
Brassica rapa hairy root based expression system leads to the production of highly homogenous and reproducible profiles of recombinant human alpha-L-iduronidase (2019), Plant Biotechnol. J., 17, 505-516 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Brassica rapa hairy roots	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.12	-	4-methylumbelliferyl alpha-L-iduronide	recombinant enzyme, at pH 3.5 and 37°C	Homo sapiens
0.13	-	4-methylumbelliferyl alpha-L-iduronide	Aldurazyme, at pH 3.5 and 37°C	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
lysosome	-	Homo sapiens	5764	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P35475	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	the enzyme has 6 N-glycosylation sites	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
Eshmuno S column chromatography and Toyopearl phenyl 650 M column chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl alpha-L-iduronide + H2O	-	Homo sapiens	4-methylumbelliferone + alpha-L-iduronic acid	-	?

Subunits

Subunits	Comment	Organism
?	x * 71000, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
Aldurazyme	commercial preparation	Homo sapiens
alpha-L-iduronidase	-	Homo sapiens
IDUA	-	Homo sapiens
rIDUA_RLT	recombinant enzyme	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
9.4	-	4-methylumbelliferyl alpha-L-iduronide	Aldurazyme, at pH 3.5 and 37°C	Homo sapiens
11.8	-	4-methylumbelliferyl alpha-L-iduronide	recombinant enzyme, at pH 3.5 and 37°C	Homo sapiens

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Release 2023.1 (January 2023)