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Literature summary for 3.2.1.75 extracted from
- Heterologous expression and characterization of a beta-1,6-glucanase from Aspergillus fumigatus (2009), Appl. Microbiol. Biotechnol., 82, 663-669.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into expression/secretion vector pINA1296+FLAG, expressed in Yarrowia lipolytica strain Po1g: MatA, leu2-270, ura3-302::pBR322-URA3, xpr2-322, axp-2, which contains an integrated pBR322 plasmid as a docking platform | Aspergillus fumigatus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
- |
Aspergillus fumigatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus fumigatus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| FLAG-tagged Neg1 protein purified by batch adsorption | Aspergillus fumigatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| laminarin + H2O | only 2% of activity at 45°C | Aspergillus fumigatus | ? | - |
? |  |
| additional information | is active on Candida albicans cell walls, is able to release a GPI-anchored cell wall protein linked to the beta-1,6-glucan polymer through a remnant of its anchor. No activity with beta-1,3-glucan, dextran and amylose | Aspergillus fumigatus | ? | - |
? | |
| pustulan + H2O | - |
Aspergillus fumigatus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-1,6-glucanase | - |
Aspergillus fumigatus |
| Neg1 | - |
Aspergillus fumigatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Aspergillus fumigatus |
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Release 2023.1 (January 2023)
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