Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.74 extracted from

  • Jeong, W.S.; Seo, D.H.; Jung, J.H.; Jung, D.H.; Lee, D.W.; Park, Y.S.; Park, C.
    Enzymatic characteristics of a highly thermostable beta-(1-4)-glucanase from Fervidobacterium islandicum AW-1 (KCTC 4680) (2017), J. Microbiol. Biotechnol., 27, 271-276 .
    View publication on PubMed
Search for more literature for 3.2.1.74

Application

Application Comment Organism
biofuel production the enzyme can be used for hydrolysis of cellulosic and hemicellulosic biomass substrates for biofuel production Fervidobacterium islandicum

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Fervidobacterium islandicum

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 28.6% residual activity at 5 mM Fervidobacterium islandicum
Co2+ 90% residual activity at 5 mM Fervidobacterium islandicum
Cu2+ 34.7% residual activity at 5 mM Fervidobacterium islandicum
EDTA 17.6% residual activity at 5 mM Fervidobacterium islandicum
Fe2+ 69.1% residual activity at 5 mM Fervidobacterium islandicum
Na+ 92.8% residual activity at 5 mM Fervidobacterium islandicum
SDS 45.7% residual activity at 0.5% (w/v) Fervidobacterium islandicum
Triton X-100 98.6% residual activity at 0.5% (w/v) Fervidobacterium islandicum
Zn2+ 65.1% residual activity at 5 mM Fervidobacterium islandicum

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 122% activity at 5 mM Fervidobacterium islandicum
Ca2+ 105.7% activity at 5 mM Fervidobacterium islandicum
Mn2+ 108.3% activity at 5 mM Fervidobacterium islandicum
additional information not affected by Mg2+ Fervidobacterium islandicum
Ni2+ 116.2% activity at 5 mM Fervidobacterium islandicum

Organism

Organism UniProt Comment Textmining
Fervidobacterium islandicum
-
 KCTC 4680
-
Fervidobacterium islandicum AW-1
-
 KCTC 4680
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Fervidobacterium islandicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl cellobioside + H2O 2756% activity compared to carboxymethyl cellulose Fervidobacterium islandicum 4-nitrophenol + cellobiose
-
 ?
4-nitrophenyl cellobioside + H2O 2756% activity compared to carboxymethyl cellulose Fervidobacterium islandicum AW-1 4-nitrophenol + cellobiose
-
 ?
avicel + H2O very low activity Fervidobacterium islandicum ?
-
 ?
avicel + H2O very low activity Fervidobacterium islandicum AW-1 ?
-
 ?
barley beta-D-glucan + H2O strongest activity (15525% activity compared to carboxymethyl cellulose) Fervidobacterium islandicum D-glucose
-
 ?
cellohexaose + H2O
-
 Fervidobacterium islandicum cellobiose + cellotriose
-
 ?
cellopentaose + H2O
-
 Fervidobacterium islandicum cellobiose + cellotriose
-
 ?
cellotetraose + H2O
-
 Fervidobacterium islandicum 2 cellobiose
-
 ?
cellotetraose + H2O
-
 Fervidobacterium islandicum AW-1 2 cellobiose
-
 ?
galactomannan + H2O 7363% activity compared to carboxymethyl cellulose Fervidobacterium islandicum ?
-
 ?
galactomannan + H2O 7363% activity compared to carboxymethyl cellulose Fervidobacterium islandicum AW-1 ?
-
 ?
hydroxyethyl cellulose + H2O 220% activity compared to carboxymethyl cellulose Fervidobacterium islandicum ?
-
 ?
additional information no activity with soluble starch, laminarin, and mannan Fervidobacterium islandicum ?
-
 ?
additional information no activity with soluble starch, laminarin, and mannan Fervidobacterium islandicum AW-1 ?
-
 ?
oat spelt xylan + H2O 57% activity compared to carboxymethyl cellulose Fervidobacterium islandicum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Fervidobacterium islandicum
? x * 41400, calculated from amino acid sequence Fervidobacterium islandicum

Synonyms

Synonyms Comment Organism
beta-(1-4)-glucanase
-
 Fervidobacterium islandicum
FiG
-
 Fervidobacterium islandicum
NA23_08975
-
 Fervidobacterium islandicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80 90
-
 Fervidobacterium islandicum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 100 about 30% activity at 50°C about 55% activity at 60°C, about 80% activity at 70°C, 100% activity at 80-90°C, about 30% activity at 100°C Fervidobacterium islandicum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70 90 the hydrolyzing activity is not diminished up to 10 days at 70°C or 75°C. The half-lives of the recombinant enzyme are about 200 and 113 h at 80°C and 85°C, respectively. The enzyme activity is drastically reduced at 90°C Fervidobacterium islandicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Fervidobacterium islandicum