Literature summary for 3.2.1.74 extracted from

Nguyen, N.P.; Lee, K.M.; Lee, K.M.; Kim, I.W.; Kim, Y.S.; Jeya, M.; Lee, J.K.
One-step purification and characterization of a beta-1,4-glucosidase from a newly isolated strain of Stereum hirsutum (2010), Appl. Microbiol. Biotechnol., 87, 2107-2116.

Search for more literature for 3.2.1.74

Activating Compound

Activating Compound	Comment	Organism	Structure
ethanol	5% (v/v) ethanol increases the enzyme activity about 30%	Stereum hirsutum
methanol	5% (v/v) methanol increases the enzyme activity about 30%	Stereum hirsutum
additional information	thiol groups are not essential for catalytic activity, the enzyme is not affected by Tween 20	Stereum hirsutum

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	competitive inhibition, 26.7% residual activity at 10 mM	Stereum hirsutum
D-glucose	D-glucose inhibition is competitive for 4-nitrophenyl beta-D-glucopyranoside	Stereum hirsutum
Fe3+	80.5% residual activity at 10 mM	Stereum hirsutum
Hg2+	competitive inhibition, 4.3% residual activity at 10 mM	Stereum hirsutum
additional information	not inhibited by EDTA, N-ethylmaleimide, beta-mercaptoethanol or cysteine. Ca2+, Co2+, Mg2+, Mn2+, and Zn2+ have no inhibitory effect	Stereum hirsutum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.5	-	4-nitrophenyl beta-D-glucopyranoside	in 100 mM sodium acetate buffer (pH 4.5), at 65°C	Stereum hirsutum
86	-	cellobiose	in 100 mM sodium acetate buffer (pH 4.5), at 65°C	Stereum hirsutum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	divalent cations are not required for enzyme activation	Stereum hirsutum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
98000	-	8 * 98000, SDS-PAGE	Stereum hirsutum
780000	-	gel filtration	Stereum hirsutum

Organism

Organism	UniProt	Comment	Textmining
Stereum hirsutum	-	-	-
Stereum hirsutum SKU512	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	-	Stereum hirsutum

Purification (Commentary)

Purification (Comment)	Organism
Superdex 200 gel filtration	Stereum hirsutum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-nitrophenyl beta-cellobioside + H2O	3.1% activity compared to 4-nitrophenyl beta-D-glucopyranoside	Stereum hirsutum	2-nitrophenol + beta-cellobiose	-	?
2-nitrophenyl beta-D-glucopyranoside + H2O	25.2% activity compared to 4-nitrophenyl beta-D-glucopyranoside	Stereum hirsutum	2-nitrophenol + D-glucopyranose	-	?
2-nitrophenyl beta-D-glucopyranoside + H2O	25.2% activity compared to 4-nitrophenyl beta-D-glucopyranoside	Stereum hirsutum SKU512	2-nitrophenol + D-glucopyranose	-	?
4-nitrophenyl beta-cellobioside + H2O	6.5% activity compared to 4-nitrophenyl beta-D-glucopyranoside	Stereum hirsutum	4-nitrophenol + beta-cellobiose	-	?
4-nitrophenyl beta-D-galactopyranoside + H2O	1.2% activity compared to 4-nitrophenyl beta-D-glucopyranoside	Stereum hirsutum	4-nitrophenol + beta-D-galactopyranose	-	?
4-nitrophenyl beta-D-glucopyranoside + H2O	100% activity	Stereum hirsutum	4-nitrophenol + D-glucopyranose	-	?
4-nitrophenyl beta-D-glucopyranoside + H2O	100% activity	Stereum hirsutum SKU512	4-nitrophenol + D-glucopyranose	-	?
cellobiose + H2O	0.96% activity compared to 4-nitrophenyl beta-D-glucopyranoside	Stereum hirsutum	2 beta-D-glucose	-	?
cellobiose + H2O	0.96% activity compared to 4-nitrophenyl beta-D-glucopyranoside	Stereum hirsutum SKU512	2 beta-D-glucose	-	?
additional information	no activity with cellotriose, cellotetraose, cellopentaose, carboxy-methylcellulose, xylan, laminarin, avicel (2% w/v), 4-nitrophenyl beta-D-mannopyranoside, and 4-nitrophenyl alpha-D-glucopyranoside	Stereum hirsutum	?	-	?
additional information	the enzyme is also able to promote transglycosylation using methanol as an acceptor and glucose as a donor	Stereum hirsutum	?	-	?
additional information	no activity with cellotriose, cellotetraose, cellopentaose, carboxy-methylcellulose, xylan, laminarin, avicel (2% w/v), 4-nitrophenyl beta-D-mannopyranoside, and 4-nitrophenyl alpha-D-glucopyranoside	Stereum hirsutum SKU512	?	-	?
additional information	the enzyme is also able to promote transglycosylation using methanol as an acceptor and glucose as a donor	Stereum hirsutum SKU512	?	-	?

Subunits

Subunits	Comment	Organism
homooctamer	8 * 98000, SDS-PAGE	Stereum hirsutum

Synonyms

Synonyms	Comment	Organism
beta-1,4-glucosidase	-	Stereum hirsutum
Bgl	-	Stereum hirsutum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
65	-	-	Stereum hirsutum

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
60	70	85% and 91% of the maximum activity at 60°C and 70°C, respectively	Stereum hirsutum

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	70	at 70°C, 65°C, 60°C, and 50°C the enzyme retains 50% of the initial activity after 2, 10, 16, and 35 min, respectively	Stereum hirsutum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
281	-	cellobiose	in 100 mM sodium acetate buffer (pH 4.5), at 65°C	Stereum hirsutum
4945	-	4-nitrophenyl beta-D-glucopyranoside	in 100 mM sodium acetate buffer (pH 4.5), at 65°C	Stereum hirsutum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.5	-	-	Stereum hirsutum

pH Range

pH Minimum	pH Maximum	Comment	Organism
4	5	84% and 86% of the maximum activity appears at pH 4.0 and 5.0, respectively	Stereum hirsutum

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
2	5.5	after 24 h of incubation at 20°C, the enzyme is fairly stable at the acidic pH ranging from 3.0 to 5.5. About 15% and 76% of activity remain after incubation at pH 2.0 and 2.5, respectively	Stereum hirsutum

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
29	-	D-glucose	in 100 mM sodium acetate buffer (pH 4.5), at 65°C	Stereum hirsutum

pI Value

Organism	Comment	pI Value Maximum	pI Value
Stereum hirsutum	isoelectric focusing	-	4.9

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Release 2023.1 (January 2023)