Application | Comment | Organism |
---|---|---|
analysis | tandem mass spectrometry to compare gas-phase and solution binding of small-molecule inhibitors to the wild type and three mutant forms of Cex | Cellulomonas fimi |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Cellulomonas fimi |
Protein Variants | Comment | Organism |
---|---|---|
N44A | based on the pUC12-1.1Cex PTIS plasmid | Cellulomonas fimi |
Q87M | based on the pUC12-1.1Cex PTIS plasmid | Cellulomonas fimi |
Q87Y | based on the pUC12-1.1Cex PTIS plasmid | Cellulomonas fimi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
xylobiosyl-deoxynojirimycin | X2DNJ | Cellulomonas fimi | |
xylobiosyl-isofagomine | X2IF | Cellulomonas fimi | |
xylobiosyl-isofagomine lactam | X2IL | Cellulomonas fimi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cellulomonas fimi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
catalytic and cellulose binding domains by cellulose FPLC column chromatography, ultrafiltration and papain treatment | Cellulomonas fimi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,4-xylan + H2O | - |
Cellulomonas fimi | ? | - |
? | |
xylan + H2O | - |
Cellulomonas fimi | ? | - |
? | |
xylo-oligosaccharides + H2O | - |
Cellulomonas fimi | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cex | - |
Cellulomonas fimi |
glucan 1,4-beta-glucosidase | - |
Cellulomonas fimi |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00013 | - |
xylobiosyl-isofagomine | wild type | Cellulomonas fimi | |
0.00034 | - |
xylobiosyl-isofagomine lactam | wild type | Cellulomonas fimi | |
0.00039 | - |
xylobiosyl-isofagomine | Q87M mutant | Cellulomonas fimi | |
0.0012 | - |
xylobiosyl-isofagomine | Q87Y mutant | Cellulomonas fimi | |
0.0021 | - |
xylobiosyl-isofagomine lactam | Q87M mutant | Cellulomonas fimi | |
0.0058 | - |
xylobiosyl-deoxynojirimycin | wild type | Cellulomonas fimi | |
0.0071 | - |
xylobiosyl-isofagomine | N44A mutant | Cellulomonas fimi | |
0.011 | - |
xylobiosyl-isofagomine lactam | Q87Y mutant | Cellulomonas fimi | |
0.018 | - |
xylobiosyl-deoxynojirimycin | Q87M mutant | Cellulomonas fimi | |
0.024 | - |
xylobiosyl-isofagomine lactam | N44A mutant | Cellulomonas fimi | |
0.062 | - |
xylobiosyl-deoxynojirimycin | Q87Y mutant | Cellulomonas fimi | |
0.18 | - |
xylobiosyl-deoxynojirimycin | N44A mutant | Cellulomonas fimi |