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Literature summary for 3.2.1.74 extracted from

  • Tesic, M.; Wicki, J.; Poon, D.K.; Withers, S.G.; Douglas, D.J.
    Gas phase noncovalent protein complexes that retain solution binding properties: Binding of xylobiose inhibitors to the beta-1, 4 exoglucanase from Cellulomonas fimi (2007), J. Am. Soc. Mass Spectrom., 18, 64-73.
    View publication on PubMed

Application

Application Comment Organism
analysis tandem mass spectrometry to compare gas-phase and solution binding of small-molecule inhibitors to the wild type and three mutant forms of Cex Cellulomonas fimi

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Cellulomonas fimi

Protein Variants

Protein Variants Comment Organism
N44A based on the pUC12-1.1Cex PTIS plasmid Cellulomonas fimi
Q87M based on the pUC12-1.1Cex PTIS plasmid Cellulomonas fimi
Q87Y based on the pUC12-1.1Cex PTIS plasmid Cellulomonas fimi

Inhibitors

Inhibitors Comment Organism Structure
xylobiosyl-deoxynojirimycin X2DNJ Cellulomonas fimi
xylobiosyl-isofagomine X2IF Cellulomonas fimi
xylobiosyl-isofagomine lactam X2IL Cellulomonas fimi

Organism

Organism UniProt Comment Textmining
Cellulomonas fimi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
catalytic and cellulose binding domains by cellulose FPLC column chromatography, ultrafiltration and papain treatment Cellulomonas fimi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,4-xylan + H2O
-
Cellulomonas fimi ?
-
?
xylan + H2O
-
Cellulomonas fimi ?
-
?
xylo-oligosaccharides + H2O
-
Cellulomonas fimi ?
-
?

Synonyms

Synonyms Comment Organism
Cex
-
Cellulomonas fimi
glucan 1,4-beta-glucosidase
-
Cellulomonas fimi

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00013
-
xylobiosyl-isofagomine wild type Cellulomonas fimi
0.00034
-
xylobiosyl-isofagomine lactam wild type Cellulomonas fimi
0.00039
-
xylobiosyl-isofagomine Q87M mutant Cellulomonas fimi
0.0012
-
xylobiosyl-isofagomine Q87Y mutant Cellulomonas fimi
0.0021
-
xylobiosyl-isofagomine lactam Q87M mutant Cellulomonas fimi
0.0058
-
xylobiosyl-deoxynojirimycin wild type Cellulomonas fimi
0.0071
-
xylobiosyl-isofagomine N44A mutant Cellulomonas fimi
0.011
-
xylobiosyl-isofagomine lactam Q87Y mutant Cellulomonas fimi
0.018
-
xylobiosyl-deoxynojirimycin Q87M mutant Cellulomonas fimi
0.024
-
xylobiosyl-isofagomine lactam N44A mutant Cellulomonas fimi
0.062
-
xylobiosyl-deoxynojirimycin Q87Y mutant Cellulomonas fimi
0.18
-
xylobiosyl-deoxynojirimycin N44A mutant Cellulomonas fimi