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Literature summary for 3.2.1.74 extracted from

  • Hamada, N.; Kodaira, R.; Nogawa, M.; Shinji, K.; Ito, R.; Amano, Y.; Shimosaka, M.; Kanda, T.; Okazaki, M.
    Role of cellulose-binding domain of exocellulase I from white rot basidiomycete Irpex lacteus (2001), J. Biosci. Bioeng., 91, 359-362.
    View publication on PubMed
Search for more literature for 3.2.1.74

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 P-42, SDS-PAGE Irpex lacteus
53000
-
 Ex-1, SDS-PAGE Irpex lacteus

Organism

Organism UniProt Comment Textmining
Irpex lacteus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification Papain digestion of native Ex-1, core fragment obtaining Irpex lacteus

Purification (Commentary)

Purification (Comment) Organism
gel filtration Irpex lacteus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0011
-
 P-42, cotton, pH 5, at 30ºC Irpex lacteus
0.0012
-
 P-42, avicel, pH 5, at 30ºC Irpex lacteus
0.0021
-
 P-42, bacterial cellulose, pH 5, at 30ºC Irpex lacteus
0.0142
-
 Ex-1, cotton, pH 5, at 30ºC Irpex lacteus
0.0145
-
 Ex-1, bacterial cellulose, pH 5, at 30ºC Irpex lacteus
0.0209
-
 Ex-1, avicel, pH 5, at 30ºC Irpex lacteus
0.0243
-
 Ex-1, 4-methylumbelliferyl-beta-D-lactoside, pH 5, at 30ºC Irpex lacteus
0.0244
-
 P-42, 4-methylumbelliferyl-beta-D-lactoside, pH 5, at 30ºC Irpex lacteus
0.026
-
 Ex-1, carboxymethyl cellulose, pH 5, at 30ºC Irpex lacteus
0.0311
-
 P-42, carboxymethyl cellulose, pH 5, at 30ºC Irpex lacteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-beta-D-lactoside + H2O
-
 Irpex lacteus 4-methylumbelliferone + D-lactose
-
 ?
avicel + H2O
-
 Irpex lacteus ?
-
 ?
bacterial cellulose + H2O
-
 Irpex lacteus ?
-
 ?
carboxymethyl cellulose + H2O
-
 Irpex lacteus ?
-
 ?
cotton + H2O
-
 Irpex lacteus ?
-
 ?

Synonyms

Synonyms Comment Organism
Ex-1 native enzyme Irpex lacteus
exocellulase I
-
 Irpex lacteus
P-42 core fragment Irpex lacteus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 Ex-1, carboxymethyl cellulose as substrate Irpex lacteus
50
-
 P-42, carboxymethyl cellulose as substrate Irpex lacteus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 40 P-42, pH 5.0, retains more than 80% of its maximum activity Irpex lacteus
25 55 Ex-1, pH 5.0, retains more than 80% of its maximum activity Irpex lacteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 Ex-1, carboxymethyl cellulose as substrate Irpex lacteus
5
-
 P-42, carboxymethyl cellulose as substrate Irpex lacteus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 7 Ex-1, retains more than 80% of its maximum activity Irpex lacteus
5 6 P-42, retains more than 70% of its maximum activity Irpex lacteus