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Literature summary for 3.2.1.73 extracted from

  • Mao, S.; Gao, P.; Lu, Z.; Lu, F.; Zhang, C.; Zhao, H.; Bie, X.
    Engineering of a thermostable beta-1,3-1,4-glucanase from Bacillus altitudinis YC-9 to improve its catalytic efficiency (2016), J. Sci. Food Agric., 96, 109-115 .
    View publication on PubMed
Search for more literature for 3.2.1.73

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus altitudinis

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular modeling of structures of wild-type and mutant K142N/Q203L/N214D does not reveal obvious differences in structure Bacillus altitudinis

Protein Variants

Protein Variants Comment Organism
K142N/Q203L/N214D mutation leads to increased activity and decreased optimal pH, while the same thermostability is maintained Bacillus altitudinis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 lichenan Km value 4 mg/ml for wild-type, 3.21 mg/ml for mutant K142N/Q203L/N214D Bacillus altitudinis

Organism

Organism UniProt Comment Textmining
Bacillus altitudinis K7XFI7
-
-
Bacillus altitudinis YC-9 K7XFI7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lichenan + H2O
-
 Bacillus altitudinis ?
-
 ?
lichenan + H2O
-
 Bacillus altitudinis YC-9 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 27000, SDS-PAGE, recombinant protein Bacillus altitudinis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
 both wild-type and mutant K142N/Q203L/N214D Bacillus altitudinis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 2 h, more than 85% residual activity, both wild-type and mutant K142N/Q203L/N214D Bacillus altitudinis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 mutant K142N/Q203L/N214D Bacillus altitudinis
6 7 wild-type Bacillus altitudinis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 4 h, more than 90% of maximum activtiy Bacillus altitudinis