Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.73 extracted from

  • Elgharbi, F.; Ben Hlima, H.; Ameri, R.; Bejar, S.; Hmida-Sayari, A.
    A trimeric and thermostable lichenase from B. pumilus US570 strain Biochemical and molecular characterization (2017), Int. J. Biol. Macromol., 95, 273-280 .
    View publication on PubMed
Search for more literature for 3.2.1.73

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular modeling of structure. Residues T2, S5,Y7, N14, S44, N78, S143, Y144, T146, D161, Q163, W209 and K213 are implicated in the contact between two monomers Bacillus pumilus

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 5 mM, 50% residual activity Bacillus pumilus
Mg2+ 5 mM, 84% residual activity Bacillus pumilus
Mn2+ 5 mM, 25% residual activity Bacillus pumilus
additional information not inhibitory: EDTA at 5 mM Bacillus pumilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 lichenan Km value 1.82 mg/ml, pH 6.0, 55°C Bacillus pumilus
additional information
-
 Barley beta-glucan Km value 2.23 mg/ml, pH 6.0, 55°C Bacillus pumilus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Bacillus pumilus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 5 mM, 117% of initial activity Bacillus pumilus
Zn2+ 5 mM, 106% of initial activity Bacillus pumilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 PAGE and gel filtration Bacillus pumilus

Organism

Organism UniProt Comment Textmining
Bacillus pumilus A0A0F6QU36
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
70.4
-
 substrate lichenan, pH 6.0, 55°C Bacillus pumilus
161
-
 substrate barley beta-glucan, pH 6.0, 55°C Bacillus pumilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
barley beta-glucan + H2O
-
 Bacillus pumilus ?
-
 ?
lichenan + H2O
-
 Bacillus pumilus ?
-
 ?
additional information no substrates: laminarin and carboxymethylcellulose Bacillus pumilus ?
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 25000, SDS-PAGE Bacillus pumilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Bacillus pumilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 65 more than 80% of maximum activity Bacillus pumilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 half-life 30 min Bacillus pumilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2613
-
 lichenan pH 6.0, 55°C Bacillus pumilus
5750
-
 Barley beta-glucan pH 6.0, 55°C Bacillus pumilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Bacillus pumilus

pH Range

pH Minimum pH Maximum Comment Organism
3 9 more than 50% of maximum activity Bacillus pumilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
 lichenan kcat/Km value 1436 ml/mg/s, pH 6.0, 55°C Bacillus pumilus
additional information
-
 Barley beta-glucan kcat/Km value 2583 ml/mg/s, pH 6.0, 55°C Bacillus pumilus