Crystallization (Comment) | Organism |
---|---|
homology modeling of structure. Amino acid residues expected to form hydrogen bond with beta-glucan, nucleophile Glu134, the acid/base catalyst Glu138, Tyr152 and water mediate hydrogen bonds with Asn55, Gln148, Asn150, Glu160, and Asn211, are found in the active site cleft | Bacillus sp. SJ-10 |
Protein Variants | Comment | Organism |
---|---|---|
K48A | mutation enhances catalytic efficiency, thermostability and halostability | Bacillus sp. SJ-10 |
K48L | mutation enhances catalytic efficiency, thermostability and halostability | Bacillus sp. SJ-10 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
Barley beta-glucan | Km value of wild-type 2.1 mg/ml, of mutant K48A 1.6 mg/ml, of mutant K48L 1.4 mg/ml, respectively, pH 6.0, 50°C | Bacillus sp. SJ-10 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. SJ-10 | I1W007 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
barley beta-glucan + H2O | - |
Bacillus sp. SJ-10 | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
half-life of wild-type 46.2 min, of mutant K48A 346 min, of mutant K48L 138 min, respectively | Bacillus sp. SJ-10 |
70 | - |
half-life of wild-type 4.5 min, of mutant K48A 30.5 min, of mutant K48L 15.1 min, respectively | Bacillus sp. SJ-10 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.45 | - |
Barley beta-glucan | wild-type, pH 6.0, 50°C | Bacillus sp. SJ-10 | |
7.45 | - |
Barley beta-glucan | mutant K48A, pH 6.0, 50°C | Bacillus sp. SJ-10 | |
7.45 | - |
Barley beta-glucan | mutant K48L, pH 6.0, 50°C | Bacillus sp. SJ-10 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
Barley beta-glucan | kcat/Km value of wild-type 3.6 ml/mg/s, of mutant K48A 4.7 ml/mg/s, of mutant K48L 5.2 ml/mg/s, respectively, pH 6.0, 50°C | Bacillus sp. SJ-10 |