Literature summary for 3.2.1.73 extracted from

Lafond, M.; Sulzenbacher, G.; Freyd, T.; Henrissat, B.; Berrin, J.G.; Garron, M.L.
The quaternary structure of a glycoside hydrolase dictates specificity toward beta-glucans (2016), J. Biol. Chem., 291, 7183-7194.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
the structure of Gluc5_26A adopts a stable trimeric quaternary structure also observable in solution. The N-terminal region protrudes into the active site of an adjacent monomer. The N-terminus governs the substrate specificity of Gluc5_26A. Its deletion opens the enzyme cleft at the -3 subsite and turns the enzyme into an endo-beta(1,4)-glucanase	Saccharophagus degradans

Protein Variants

Protein Variants	Comment	Organism
additional information	deletion of the N-terminal sequence up to residue Ser38. The mutant is devoid of the helix-turn motif interacting with residues of the substrate-binding cleft at the level of subsite -3. The mutation uncovers the enzyme cleft at the -3 subsite and turns the enzyme into an endo-beta(1,4)-glucanase	Saccharophagus degradans

General Stability

General Stability	Organism
the enzyme is able to conserve its activity after a drastic treatment with 500 mM NaOH, and the only way to stop enzyme activity is treatment with 8 M urea	Saccharophagus degradans

Organism

Organism	UniProt	Comment	Textmining
Saccharophagus degradans	Q21KE5	-	-
Saccharophagus degradans DSM 17024	Q21KE5	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
barley beta-glucan + H2O	-	Saccharophagus degradans	Glc-beta(1,4)-Glc-beta(1,3)-Glc-beta(1,4)-Glc + Glc-beta(1,3)-Glc-beta(1,4)-Glc-OH	final products	?
barley beta-glucan + H2O	-	Saccharophagus degradans DSM 17024	Glc-beta(1,4)-Glc-beta(1,3)-Glc-beta(1,4)-Glc + Glc-beta(1,3)-Glc-beta(1,4)-Glc-OH	final products	?
cellopentaose + H2O	-	Saccharophagus degradans	cellobiose + D-glucose	with N-terminal deletion mutant, products are cellotriose and cellobiose	?
cellopentaose + H2O	-	Saccharophagus degradans DSM 17024	cellobiose + D-glucose	with N-terminal deletion mutant, products are cellotriose and cellobiose	?
Glc-beta(1,4)-Glc-beta(1,4)-Glc-beta(1,3)-Glc + H2O	-	Saccharophagus degradans	cellobiose + laminaribiose	with N-terminal deletion mutant, products are cellotriose and D-glucose	?
Glc-beta(1,4)-Glc-beta(1,4)-Glc-beta(1,3)-Glc + H2O	-	Saccharophagus degradans DSM 17024	cellobiose + laminaribiose	with N-terminal deletion mutant, products are cellotriose and D-glucose	?
lichenan + H2O	-	Saccharophagus degradans	cellobiose + Glc-beta(1,4)-Glc-beta(1,3)-Glc-beta(1,4)-Glc + Glc-beta(1,3)-Glc-beta(1,4)-Glc-OH	final products	?
lichenan + H2O	-	Saccharophagus degradans DSM 17024	cellobiose + Glc-beta(1,4)-Glc-beta(1,3)-Glc-beta(1,4)-Glc + Glc-beta(1,3)-Glc-beta(1,4)-Glc-OH	final products	?

Synonyms

Synonyms	Comment	Organism
Cel5F	-	Saccharophagus degradans
Gluc5_26A	-	Saccharophagus degradans

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	-	Saccharophagus degradans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Saccharophagus degradans

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Release 2023.1 (January 2023)