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Literature summary for 3.2.1.73 extracted from
- Potential application of two thermostable lichenases from a newly isolated Bacillus licheniformis UEB CF: purification and characterization (2012), Process Biochem., 47, 509-516.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | inhibits isozyme EG1 by 35%, and isozyme EG2 also slightly, at 2.5 mM | Bacillus licheniformis |  |
| Cu2+ | inhibits isozyme EG1 by 28%, but slightly activates isozyme EG2 at 2.5 mM | Bacillus licheniformis | |
| Fe2+ | inhibits isozyme EG1 by 50% at 2.5 mM | Bacillus licheniformis | |
| H2O2 | at 0.5% w/v hydrogen peroxide, isozymes EG1 and EG2 retain 82.8% and 90.28% of their activities, respectively. At 1% w/v hydrogen peroxide, isozyme EG2 retains 60% activity, while isozyme EG1 is completely inhibited | Bacillus licheniformis | |
| Hg2+ | complete inhibition of both isozymes at 2.5 mM | Bacillus licheniformis | |
| additional information | the isozymes are highly stable in the presence of non-ionic surfactants such as Tween 80 and Triton X-100 | Bacillus licheniformis | |
| SDS | the strong anionic surfactant at 0.1% and 0.5% causes a moderate inhibition of 27.5% and 30.7% of isozyme EG2 activity. Isozyme EG1 is highly stable against SDS and retains 100% of its activity in the presence of 0.1% and 0.5% SDS | Bacillus licheniformis | |
| sodium perborate | isozymes EG1 and EG2 retain 58% and 66.8% of their activities after 1 h of incubation at 40°C in the presence of 0.2% w/v sodium perborate, respectively | Bacillus licheniformis | |
| Zn2+ | 78% inhibition of isozyme EG1, poor inhibition of isozyme EG2 | Bacillus licheniformis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | 2.1 mg/ml for isozyme EG1 and 1.8 mg/ml for isozyme EG2 with lichenan at pH 5.0, 50°C | Bacillus licheniformis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | enhances the isozyme EG1 and EG2 activities by 32.7% and 12%, respectively, at 2.5 mM. Ca2+ enhances the substrate binding affinity of the enzyme and stabilize the conformation of the catalytic site | Bacillus licheniformis | |
| Cu2+ | inhibits isozyme EG1 by 35%, but slightly activates isozyme EG2 at 2.5 mM | Bacillus licheniformis | |
| Fe2+ | slightly activates isozyme EG2 at 2.5 mM | Bacillus licheniformis | |
| additional information | poor effects on both isozymes by Mg2+ and EDTA at 2.5 mM | Bacillus licheniformis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
1 * 30000, isozyme EG1, SDS-PAGE, 1 * 50000, isozyme EG2, SDS-PAGE | Bacillus licheniformis |
| 50000 | - |
1 * 30000, isozyme EG1, SDS-PAGE, 1 * 50000, isozyme EG2, SDS-PAGE | Bacillus licheniformis |
| 55000 | - |
isozyme EG2, native PAGE | Bacillus licheniformis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus licheniformis | - |
isozymes EG1 and EG2 | - |
| Bacillus licheniformis UEB CF | - |
isozymes EG1 and EG2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular isozymes EG1 and EG2 0.24fold and 11.6fold, respectively, from cell-free culture supernatant by dialysis and concentration with PEG 400, followed by anion exchange chromatography and gel filtration, both isozymes to homogeneity | Bacillus licheniformis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15.6 | - |
isozyme EG1, pH 5.0, 50°C, substrate lichenan | Bacillus licheniformis |
| 767 | - |
isozyme EG2, pH 5.0, 50°C, substrate lichenan | Bacillus licheniformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| barley beta-glucan + H2O | - |
Bacillus licheniformis | 3-O-beta-cellobiosyl-D-glucose + 3-O-beta-cellotriosyl-D-glucose | oligosaccharides, mainly trisaccharide and tetrasaccharide | ? | |
| barley beta-glucan + H2O | - |
Bacillus licheniformis UEB CF | 3-O-beta-cellobiosyl-D-glucose + 3-O-beta-cellotriosyl-D-glucose | oligosaccharides, mainly trisaccharide and tetrasaccharide | ? | |
| lichenan + H2O | - |
Bacillus licheniformis | 3-O-beta-cellobiosyl-D-glucose + 3-O-beta-cellotriosyl-D-glucose | oligosaccharides, mainly trisaccharide and tetrasaccharide | ? | |
| lichenan + H2O | - |
Bacillus licheniformis UEB CF | 3-O-beta-cellobiosyl-D-glucose + 3-O-beta-cellotriosyl-D-glucose | oligosaccharides, mainly trisaccharide and tetrasaccharide | ? | |
| additional information | no activity with carboxymethyl cellulose and laminarin, isozymes EG1 and EG2 exhibit no activity for pure beta-1,3 bond and for pure beta-1,4 glucan forms | Bacillus licheniformis | ? | - |
? | |
| additional information | no activity with carboxymethyl cellulose and laminarin, isozymes EG1 and EG2 exhibit no activity for pure beta-1,3 bond and for pure beta-1,4 glucan forms | Bacillus licheniformis UEB CF | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 30000, isozyme EG1, SDS-PAGE, 1 * 50000, isozyme EG2, SDS-PAGE | Bacillus licheniformis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EG1 | - |
Bacillus licheniformis |
| Lichenase | - |
Bacillus licheniformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
isozyme EG2 | Bacillus licheniformis |
| 70 | - |
isozyme EG1 | Bacillus licheniformis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 50 | purified isozymes EG1 and EG2, retaining over 90% activity after 1 h | Bacillus licheniformis |
| 60 | - |
purified isozymes EG1 and EG2, half-lives are 55 min and 5 h, respectively | Bacillus licheniformis |
| 70 | - |
purified isozymes EG1 and EG2, half-lives are 10 min and 52 min, respectively | Bacillus licheniformis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 60 | - |
lichenan | isozyme EG1, pH 5.0, 50°C | Bacillus licheniformis | |
| 708.3 | - |
lichenan | isozyme EG1, pH 5.0, 50°C | Bacillus licheniformis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
isozyme EG1 | Bacillus licheniformis |
| 7 | - |
isozyme EG2 | Bacillus licheniformis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | isozyme EG1, more than 80% of maximal activity within pH range pH 4.0-7.0, 50% at pH 10.0. Isozyme Eg2 shows over 70% of maximal activity at pH 4.0-10.0 | Bacillus licheniformis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | purified isozymes EG1 and EG2, retaining over 90% activity after 48 h, after 72 h isozyme EG1 loses 30% and isozyme EG2 15% activity | Bacillus licheniformis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | N-terminal amino acid sequences of isozymes EG1 and EG2 are GAAPIKKGTTKLN and DINGGGATLPQK, respectively | Bacillus licheniformis |
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