Application | Comment | Organism |
---|---|---|
food industry | the secretively produced beta-1,3-1,4-glucanase shows excellent thermostability up to 80°C and a wide pH range from pH 4 to pH 11 and has a potential in the food and animal feed applications | Acetivibrio thermocellus |
Cloned (Comment) | Organism |
---|---|
recombinant expression and secretion from Bacillus subtilis strain WB800 using vector pP43JM2 | Acetivibrio thermocellus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | inhibits 37% at 10 mM | Acetivibrio thermocellus | |
Fe2+ | inhibits 51% at 10 mM | Acetivibrio thermocellus | |
Mn2+ | inhibits 6.5% at 10 mM | Acetivibrio thermocellus | |
additional information | poor inhibition at 10 mM by Co2+ and EDTA | Acetivibrio thermocellus | |
Zn2+ | inhibits 15% at 10 mM | Acetivibrio thermocellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 1.65 mg/ml with beta-1,3-1,4-glucan, at pH 6.0, 70°C | Acetivibrio thermocellus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no effects by 10 mM of Na+, K+, Ca2+, and Mg2+ | Acetivibrio thermocellus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,3-1,4-glucan + H2O | Acetivibrio thermocellus | - |
? | - |
? | |
beta-1,3-1,4-glucan + H2O | Acetivibrio thermocellus DSM 1237 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | - |
- |
- |
Acetivibrio thermocellus DSM 1237 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,3-1,4-glucan + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
beta-1,3-1,4-glucan + H2O | - |
Acetivibrio thermocellus DSM 1237 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-1,3-1,4-glucanase | - |
Acetivibrio thermocellus |
LicB | - |
Acetivibrio thermocellus |
Lichenase | - |
Acetivibrio thermocellus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
recombinant enzyme | Acetivibrio thermocellus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 80 | activity range, recombinant enzyme | Acetivibrio thermocellus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
purified recombinant enzyme, pH 7.0, 40% activity remaining after 6 h | Acetivibrio thermocellus |
80 | - |
purified recombinant enzyme, pH 7.0, no activity remaining after 1 h | Acetivibrio thermocellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Acetivibrio thermocellus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | activity range, recombinant enzyme, profile overview | Acetivibrio thermocellus |