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Literature summary for 3.2.1.73 extracted from
- Purification, characterization, and heterologous expression of a thermostable beta-1,3-1,4-glucanase from Bacillus altitudinis YC-9 (2013), Appl. Biochem. Biotechnol., 169, 960-975.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, sequence comparisons, and phylogenetic analysis. Recombinant expression of the enzyme fused to the Trx-tag/His-tag/S-tag sequences in Escherichia coli strain BL21 (DE3), the enzyme is located in the cell lysate and the cell culture supernatant | Bacillus altitudinis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Bacillus altitudinis |  |
| EDTA | - |
Bacillus altitudinis | |
| Mn2+ | - |
Bacillus altitudinis | |
| Zn2+ | slightly inhibitory | Bacillus altitudinis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates slightly | Bacillus altitudinis | |
| Co2+ | activates | Bacillus altitudinis | |
| Fe2+ | activates | Bacillus altitudinis | |
| additional information | no or poor effects by K+, Na+, and Mg2+ | Bacillus altitudinis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27470 | - |
x * 27470, sequence calculation. x * 45000, recombinant tagged enzyme, SDS-PAGE | Bacillus altitudinis |
| 45000 | - |
x * 27470, sequence calculation. x * 45000, recombinant tagged enzyme, SDS-PAGE | Bacillus altitudinis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lichenan + H2O | Bacillus altitudinis | - |
? | - |
? | |
| lichenan + H2O | Bacillus altitudinis YC-9 | - |
? | - |
? | |
| additional information | Bacillus altitudinis | beta-1,3-1,4-glucanase strictly hydrolyzes beta-1,4-glycosidic linkage adjacent to a 3-O-substituted glucose residue in mixed linked beta-glucans but cannot act on beta-1,4-glucans | ? | - |
? | |
| additional information | Bacillus altitudinis YC-9 | beta-1,3-1,4-glucanase strictly hydrolyzes beta-1,4-glycosidic linkage adjacent to a 3-O-substituted glucose residue in mixed linked beta-glucans but cannot act on beta-1,4-glucans | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus altitudinis | K7XFI7 | isolated from spring silt | - |
| Bacillus altitudinis YC-9 | K7XFI7 | isolated from spring silt | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 37fold from strain YC-9 by ammonium sulfate precipitation, anion exchange chromatography, and gel filtration, recombinant enzyme from Escherichia coli strain BL21 (DE3) cell culture supernatant by ammonium sulfate precipitation, anion exchange chromatography, and gel filtration | Bacillus altitudinis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2373 | - |
purified native enzyme, pH 6.0, 65°C | Bacillus altitudinis |
| 5393 | - |
purified recombinant enzyme, pH 6.0, 65°C | Bacillus altitudinis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lichenan + H2O | - |
Bacillus altitudinis | ? | - |
? | |
| lichenan + H2O | - |
Bacillus altitudinis YC-9 | ? | - |
? | |
| additional information | beta-1,3-1,4-glucanase strictly hydrolyzes beta-1,4-glycosidic linkage adjacent to a 3-O-substituted glucose residue in mixed linked beta-glucans but cannot act on beta-1,4-glucans | Bacillus altitudinis | ? | - |
? | |
| additional information | native and recombinant enzymes are both inactive on laminarin, cellulose, xylan, or mannan | Bacillus altitudinis | ? | - |
? | |
| additional information | beta-1,3-1,4-glucanase strictly hydrolyzes beta-1,4-glycosidic linkage adjacent to a 3-O-substituted glucose residue in mixed linked beta-glucans but cannot act on beta-1,4-glucans | Bacillus altitudinis YC-9 | ? | - |
? | |
| additional information | native and recombinant enzymes are both inactive on laminarin, cellulose, xylan, or mannan | Bacillus altitudinis YC-9 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 27470, sequence calculation. x * 45000, recombinant tagged enzyme, SDS-PAGE | Bacillus altitudinis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-1,3-1,4-glucanase | - |
Bacillus altitudinis |
| Lichenase | - |
Bacillus altitudinis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
native and recombinant enzyme | Bacillus altitudinis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 80 | activity profile, overview | Bacillus altitudinis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
purified recombinant enzyme, retains more than 90% activity after 2 h | Bacillus altitudinis |
| 70 | - |
purified recombinant enzyme, retains more 75% activity after 10 min | Bacillus altitudinis |
| 80 | - |
purified recombinant enzyme, retains more 44% activity after 10 min | Bacillus altitudinis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
recombinant enzyme | Bacillus altitudinis |
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