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Literature summary for 3.2.1.73 extracted from
- Structural and catalytic roles of amino acid residues located at substrate-binding pocket in Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase (2010), Proteins Struct. Funct. Bioinform., 78, 2820-2830.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| mutant F40I, to 1.7 A resolution. Overall globular structures in the wild-type and mutant F40I enzymes do not differ | Fibrobacter succinogenes |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E11L | mutant of truncated beta-glucanase catalytic domain, residues 1-243. No discernible changes in secondary structure, more than 10fold decrease in specific activity, more than 2fold increase in KM-value, significant decrease in catalytic efficiency | Fibrobacter succinogenes |
| E47I | mutant of truncated beta-glucanase catalytic domain, residues 1-243. No discernible changes in secondary structure, more than 2fold increase in KM-value | Fibrobacter succinogenes |
| F40I | mutant of truncated beta-glucanase catalytic domain, residues 1-243. No discernible changes in secondary structure, more than 10fold decrease in specific activity, significant decrease in catalytic efficiency | Fibrobacter succinogenes |
| K64A | mutant of truncated beta-glucanase catalytic domain, residues 1-243. No discernible changes in secondary structure | Fibrobacter succinogenes |
| K64M | mutant of truncated beta-glucanase catalytic domain, residues 1-243. No discernible changes in secondary structure, more than 2fold increase in KM-value | Fibrobacter succinogenes |
| L62G | mutant of truncated beta-glucanase catalytic domain, residues 1-243. No discernible changes in secondary structure | Fibrobacter succinogenes |
| N139A | mutant of truncated beta-glucanase catalytic domain, residues 1-243. No discernible changes in secondary structure, more than 2fold increase in KM-value, significant decrease in catalytic efficiency | Fibrobacter succinogenes |
| N44L | mutant of truncated beta-glucanase catalytic domain, residues 1-243. No discernible changes in secondary structure | Fibrobacter succinogenes |
| N44Q | mutant of truncated beta-glucanase catalytic domain, residues 1-243. No discernible changes in secondary structure, more than 2fold increase in KM-value | Fibrobacter succinogenes |
| R137M | mutant of truncated beta-glucanase catalytic domain, residues 1-243. No discernible changes in secondary structure, more than 10fold decrease in specific activity, more than 2fold increase in KM-value, significant decrease in catalytic efficiency | Fibrobacter succinogenes |
| R137Q | mutant of truncated beta-glucanase catalytic domain, residues 1-243. No discernible changes in secondary structure, more than 10fold decrease in specific activity, more than 2fold increase in KM-value, significant decrease in catalytic efficiency | Fibrobacter succinogenes |
| Y42L | mutant of truncated beta-glucanase catalytic domain, residues 1-243. No discernible changes in secondary structure, more than 10fold decrease in specific activity | Fibrobacter succinogenes |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | KM-value for wild-type, substrate lichenan, 2.88 mg/ml | Fibrobacter succinogenes |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fibrobacter succinogenes | P17989 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.6 | - |
mutant E11L, 45°C, pH 5.0 | Fibrobacter succinogenes |
| 15 | 25 | mutant N139A, 45°C, pH not specified in the publication | Fibrobacter succinogenes |
| 43 | 46 | mutant N44L, 55°C, pH not specified in the publication | Fibrobacter succinogenes |
| 60 | 61 | mutant N44Q, 55°C, pH not specified in the publication | Fibrobacter succinogenes |
| 262 | - |
mutant F40I, 45°C, pH not specified in the publication | Fibrobacter succinogenes |
| 308 | - |
mutant R137Q, 40°C, pH not specified in the publication | Fibrobacter succinogenes |
| 644 | - |
mutant Y42L, 45°C, pH not specified in the publication | Fibrobacter succinogenes |
| 699 | - |
mutant R137M, 40°C, pH not specified in the publication | Fibrobacter succinogenes |
| 803 | - |
mutant L62G, 45°C, pH not specified in the publication | Fibrobacter succinogenes |
| 1986 | - |
mutant E47I, 45°C, pH not specified in the publication | Fibrobacter succinogenes |
| 3581 | - |
mutant K64A, 50°C, pH not specified in the publication | Fibrobacter succinogenes |
| 3671 | - |
mutant K64M, 50°C, pH not specified in the publication | Fibrobacter succinogenes |
| 7833 | - |
wild-type, 50°C, pH 5.0 | Fibrobacter succinogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lichenan + H2O | - |
Fibrobacter succinogenes | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TF-glucanase | - |
Fibrobacter succinogenes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
mutants R137M and R137Q | Fibrobacter succinogenes |
| 45 | - |
mutants E11L, F40I, Y42L, E47I, L62G, and N139A | Fibrobacter succinogenes |
| 50 | - |
wild-type, mutants K64A and K64M | Fibrobacter succinogenes |
| 55 | - |
mutants N44L and N44Q | Fibrobacter succinogenes |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.8 | - |
lichenan | mutant E11L, 45°C, pH 5.0 | Fibrobacter succinogenes | |
| 131 | - |
lichenan | mutant F40I, 45°C, pH not specified in the publication | Fibrobacter succinogenes | |
| 154 | - |
lichenan | mutant R137Q, 40°C, pH not specified in the publication | Fibrobacter succinogenes | |
| 322 | - |
lichenan | mutant Y42L, 45°C, pH not specified in the publication | Fibrobacter succinogenes | |
| 349 | - |
lichenan | mutant R137M, 40°C, pH not specified in the publication | Fibrobacter succinogenes | |
| 401 | - |
lichenan | mutant L62G, 45°C, pH not specified in the publication | Fibrobacter succinogenes | |
| 756 | - |
lichenan | mutant N139A, 45°C, pH not specified in the publication | Fibrobacter succinogenes | |
| 992 | - |
lichenan | mutant E47I, 45°C, pH not specified in the publication | Fibrobacter succinogenes | |
| 1788 | - |
lichenan | mutant K64A, 50°C, pH not specified in the publication | Fibrobacter succinogenes | |
| 1833 | - |
lichenan | mutant K64M, 50°C, pH not specified in the publication | Fibrobacter succinogenes | |
| 2170 | - |
lichenan | mutant N44L, 55°C, pH not specified in the publication | Fibrobacter succinogenes | |
| 3026 | - |
lichenan | mutant N44Q, 55°C, pH not specified in the publication | Fibrobacter succinogenes | |
| 3911 | - |
lichenan | wild-type, 50°C, pH 5.0 | Fibrobacter succinogenes | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
wild-type and mutant E11L | Fibrobacter succinogenes |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kcat/KM-value for wild-type, substrate lichenan, 1358 ml/mg/s | Fibrobacter succinogenes |
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