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Literature summary for 3.2.1.73 extracted from
- Grass degrading beta-1,3-1,4-D-glucanases from Bacillus subtilis GN156: purification and characterization of glucanase J1 and pJ2 possessing extremely acidic pI (2008), Appl. Biochem. Biotechnol., 149, 53-66.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | partially inhibited | Bacillus subtilis |  |
| Cu2+ | partially inhibited | Bacillus subtilis | |
| Fe2+ | slightly inhibited | Bacillus subtilis | |
| Mg2+ | slightly inhibited | Bacillus subtilis | |
| Mn2+ | partially inhibited | Bacillus subtilis | |
| additional information | not inhibited by Li+; not inhibited by Li+, not significantly by Fe2+ | Bacillus subtilis | |
| Zn2+ | partially inhibited | Bacillus subtilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
Barley beta-glucan | 1.53 mg/ml | Bacillus subtilis | |
| additional information | - |
Barley beta-glucan | 4.36 mg/ml with substrate barley beta-glucan | Bacillus subtilis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus subtilis | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
SDS-PAGE | Bacillus subtilis |
| 90000 | - |
SDS-PAGE, J2 is composed of three protein subunits of 40, 32 and 18 kDa | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Bacillus subtilis GN156 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography of epoxy-activated sepharose 6B and ultrafiltration technique | Bacillus subtilis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture supernatant | - |
Bacillus subtilis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 82.45 | - |
- |
Bacillus subtilis |
| 555.1 | - |
purified enzyme | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| barley beta-glucan + H2O | - |
Bacillus subtilis | additional information | pentose, triose, tetarose and a high molecular weight polysaccharide with 1,3 linkage | ? | |
| barley beta-glucan + H2O | - |
Bacillus subtilis | additional information | pentose, triose, tetrose and a high molecular weight polysaccharide with 1,3 linkage | ? | |
| barley beta-glucan + H2O | - |
Bacillus subtilis GN156 | additional information | pentose, triose, tetarose and a high molecular weight polysaccharide with 1,3 linkage | ? | |
| barley beta-glucan + H2O | - |
Bacillus subtilis GN156 | additional information | pentose, triose, tetrose and a high molecular weight polysaccharide with 1,3 linkage | ? | |
| additional information | no activity against carboxymethylcellulose, xylan, laminarin, dextrin | Bacillus subtilis | ? | - |
? | |
| additional information | no activity against carboxymethylcellulose, xylan, laminarin, dextrin | Bacillus subtilis GN156 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | four protein bands of approximately 40, 32, 25 and 18 kDa are resolved from pJ2 | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 1,3-1,4-beta-D-glucan-4-glucano hydrolase | - |
Bacillus subtilis |
| beta-1,3-1,4-glucanase | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Bacillus subtilis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 70 | 30 min with about 50% of activity remaining at 50°C | Bacillus subtilis |
| 20 | 70 | the remaining activity decreased to 20% at 20-40°C and is completely destroyed at 50-70°C | Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | 30 min with about 50% of activity remaining at 50°C | Bacillus subtilis |
| 20 | 40 | the remaining activity decreased to 20% at 20-40°C and is completely destroyed at 50-70°C | Bacillus subtilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.074 | - |
Barley beta-glucan | - |
Bacillus subtilis | |
| 0.091 | - |
Barley beta-glucan | - |
Bacillus subtilis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Bacillus subtilis |
| 6.5 | - |
- |
Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | with 50% of the activity remaining at pH 9 | Bacillus subtilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | with 50% of the activity remaining at pH 9 | Bacillus subtilis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bacillus subtilis | isoelectric focusing | - |
3.5 |
| Bacillus subtilis | isoelectricfocusing | - |
3.6 |
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