Any feedback?
Literature summary for 3.2.1.73 extracted from
- Characterization of a beta-glucanase produced by Rhizopus microsporus var. microsporus, and its potential for application in the brewing industry (2006), BMC Biochem., 7, 23.
Application
| Application | Comment | Organism |
|---|---|---|
| nutrition | the enzyme is able to reduce both the viscosity of the brewer mash and the filtration time, indicating its potential value for the brewing industry | Rhizopus microsporus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 0.3% residual activity at 12 mM | Rhizopus microsporus |  |
| Fe3+ | 89.6% residual activity at 12 mM | Rhizopus microsporus | |
| Mg2+ | 95.2% residual activity at 12 mM | Rhizopus microsporus | |
| Mn2+ | 62.3% residual activity at 12 mM | Rhizopus microsporus | |
| additional information | shows no sensitivity to Ca2+ and Al3+ | Rhizopus microsporus | |
| Zn2+ | 65% residual activity at 12 mM | Rhizopus microsporus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33730 | - |
mass spectrometry | Rhizopus microsporus |
| 36500 | - |
SDS-PAGE | Rhizopus microsporus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhizopus microsporus | - |
variant microsporus | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| SP-Sepharose column chromatography and Sephacryl S-100 gel filtration | Rhizopus microsporus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.197 | - |
concentrated crude extract, at 50°C, pH 5 | Rhizopus microsporus |
| 12.6 | - |
after 55.53fold purification, at 50°C, pH 5 | Rhizopus microsporus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,3-1,4-beta-glucan + H2O | - |
Rhizopus microsporus | ? | - |
? | |
| lichenan + H2O | - |
Rhizopus microsporus | ? | - |
? | |
| additional information | the enzyme shows only residual activity against 1,3-beta-glucan (laminarin) or no activity at all against 1,4-beta-glucan (cellulose), chitin, xylan, or manan | Rhizopus microsporus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 1,3-1,4-beta-glucanase | - |
Rhizopus microsporus |
| beta-glucanase | - |
Rhizopus microsporus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | - |
Rhizopus microsporus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 70 | after 2 h incubation at 50°C and 60°C, the residual activity remaines 100% and 50%, respectively, while the enzymatic activity is abolished after 3 min incubation at 70°C | Rhizopus microsporus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 5 | - |
Rhizopus microsporus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
no enzyme activity is detected at pH higher than 6 | Rhizopus microsporus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
