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Literature summary for 3.2.1.73 extracted from

  • Anish, R.; Rao, M.
    Biochemical characterization of a novel beta-1-3, 1-4 glucan 4-glucanohydrolase from Thermomonospora sp. having a single active site for lichenan and xylan (2007), Biochimie, 89, 1489-1497.
    View publication on PubMed
Search for more literature for 3.2.1.73

Inhibitors

Inhibitors Comment Organism Structure
2,4,6-Trinitrobenzenesulfonic acid
-
 Thermomonospora sp.
5,5'-dithiobis 2-nitrobenzoic acid
-
 Thermomonospora sp.
o-phthaldehyde
-
 Thermomonospora sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64120
-
 gel filtration Thermomonospora sp.
64240
-
 calculated from amino acid sequence Thermomonospora sp.
64500
-
 SDS-PAGE Thermomonospora sp.
66730
-
 MALDI-TOF mass spectrometry Thermomonospora sp.

Organism

Organism UniProt Comment Textmining
Thermomonospora sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE Sephadex A50 column chromatography and Sephacryl S-200 gel filtration Thermomonospora sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18.89
-
 enzyme from culture filtrate, using xylan as a substrate, at 50°C, pH 7.0 Thermomonospora sp.
25
-
 enzyme from culture filtrate, using lichenan as a substrate, at 50°C, pH 7.0 Thermomonospora sp.
598.6
-
 after 31.6fold purification, using xylan as a substrate, at 50°C, pH 7.0 Thermomonospora sp.
792.2
-
 after 31.6fold purification, using lichenan as a substrate, at 50°C, pH 7.0 Thermomonospora sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lichenan + H2O
-
 Thermomonospora sp. D-glucose + ?
-
 ?
additional information no activity towards avicel, laminarin, gum arabic, p-nitrophenyl glucoside, and p-nitrophenyl xylopyranoside Thermomonospora sp. ?
-
 ?
xylan + H2O
-
 Thermomonospora sp. xylose + ?
-
 ?

Synonyms

Synonyms Comment Organism
beta-1-3, 1-4 glucan 4-glucanohydrolase
-
 Thermomonospora sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Thermomonospora sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 half-life of 5 min at 80°C Thermomonospora sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Thermomonospora sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8
-
 Thermomonospora sp.

pI Value

Organism Comment pI Value Maximum pI Value
Thermomonospora sp. isoelectric focusing
-
 4.27