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Literature summary for 3.2.1.73 extracted from
- How family 26 glycoside hydrolases orchestrate catalysis on different polysaccharides: structure and activity of a Clostridium thermocellum lichenase, CtLic26A (2005), J. Biol. Chem., 280, 32761-32767.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli XL1-blue and BL21 with His-tag | Acetivibrio thermocellus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| 1.5 A resolution, native enzyme, 1.6 A resolution, enzyme-inhibitor Glc-beta-1,3-isofagomine-complex, hanging drop vapor diffusion method for the enzyme, sitting drop vapor diffusion method for the complex | Acetivibrio thermocellus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Glc-beta-1,3-isofagomine | - |
Acetivibrio thermocellus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.3 | - |
Glcbeta3Glcbeta-methylumbelliferone | pH 7.0, 37°C, 50 mM sodium phosphate buffer | Acetivibrio thermocellus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetivibrio thermocellus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| immobilized metal ion affinity chromatography, gel filtration, anion exchange chromatography | Acetivibrio thermocellus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| barley beta-glucan + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
| Glcbeta3Glcbeta-methylumbelliferone + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
| lichenan + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
| additional information | no activity against beta-1,4- or beta-1,3 homopolymers of gluco- or manno-configured polysaccharides | Acetivibrio thermocellus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CtLic26A | - |
Acetivibrio thermocellus |
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Release 2023.1 (January 2023)
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