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Literature summary for 3.2.1.73 extracted from

  • Chen, J.L.; Tsai, L.C.; Wen, T.N.; Tang, J.B.; Yuan, H.S.; Shyur, L.F.
    Directed mutagenesis of specific active site residues on Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase significantly affects catalysis and enzyme structural stability (2001), J. Biol. Chem., 276, 17895-17901.
    View publication on PubMed
Search for more literature for 3.2.1.73

Protein Variants

Protein Variants Comment Organism
D58A no enzymatic activity Fibrobacter succinogenes
D58E dramatic decrease in kcat, substrate affinity similar to wild type Fibrobacter succinogenes
D58N dramatic decrease in kcat, substrate affinity similar to wild type Fibrobacter succinogenes
E56A no enzymatic activity Fibrobacter succinogenes
E56D dramatic decrease in kcat, substrate affinity similar to wild type Fibrobacter succinogenes
E56Q no enzymatic activity Fibrobacter succinogenes
E60A no enzymatic activity Fibrobacter succinogenes
E60D dramatic decrease in kcat, substrate affinity similar to wild type Fibrobacter succinogenes
E60Q no enzymatic activity Fibrobacter succinogenes
G63A decrease in thermostability Fibrobacter succinogenes
M39F 5-fold increase in km value Fibrobacter succinogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.98
-
 lichenan pH 6.0, 40°C, mutant D58N Fibrobacter succinogenes
1.04
-
 lichenan pH 6.0, 40°C, mutant E60D Fibrobacter succinogenes
1.69
-
 lichenan pH 6.0, 40°C, mutant E56D Fibrobacter succinogenes
1.91
-
 lichenan pH 6.0, 40°C, wild type Fibrobacter succinogenes
2.1
-
 lichenan pH 6.0, 40°C, mutant D58E Fibrobacter succinogenes
3.1
-
 lichenan pH 6.0, 40°C, mutant G63A Fibrobacter succinogenes
10.3
-
 lichenan pH 6.0, 40°C, mutant M39F Fibrobacter succinogenes

Organism

Organism UniProt Comment Textmining
Fibrobacter succinogenes
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.57
-
 pH 6.0, 40°C, mutant D58E Fibrobacter succinogenes
2.43
-
 pH 6.0, 40°C, mutant D58N Fibrobacter succinogenes
2.57
-
 pH 6.0, 40°C, mutant E60D Fibrobacter succinogenes
5.75
-
 pH 6.0, 40°C, mutant E56D Fibrobacter succinogenes
502
-
 pH 6.0, 40°C, mutant G63A Fibrobacter succinogenes
545
-
 pH 6.0, 40°C, mutant M39F Fibrobacter succinogenes
1388
-
 pH 6.0, 40°C, wild type Fibrobacter succinogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lichenan + H2O
-
 Fibrobacter succinogenes ?
-
 ?