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Literature summary for 3.2.1.73 extracted from
- Characterization, cloning and sequencing of a thermostable endo-(1,3-1,4)beta-glucanase-encoding gene from an alkalophilic Bacillus brevis (1993), Appl. Microbiol. Biotechnol., 38, 507-513.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Brevibacillus brevis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no inhibition up to a concentration of 10% SDS-PAGE | Brevibacillus brevis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Brevibacillus brevis | 5737 | - |
| extracellular | - |
Brevibacillus brevis | - |
- |
| periplasmic space | - |
Brevibacillus brevis | 42597 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
x * 29000, SDS-PAGE | Brevibacillus brevis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brevibacillus brevis | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lichenan + H2O | - |
Brevibacillus brevis | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 29000, SDS-PAGE | Brevibacillus brevis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | 70 | - |
Brevibacillus brevis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 80 | 40°C: about 40% of maximal activity, 80°C: about 30% of maximal activity | Brevibacillus brevis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
1 h, 15% loss of activity | Brevibacillus brevis |
| 75 | - |
1 h, 25% loss of activity | Brevibacillus brevis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10 | - |
Brevibacillus brevis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | pH 6.0: about 55% of maximal activity, pH 11.0: about 45% of maximal activity | Brevibacillus brevis |
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Release 2023.1 (January 2023)
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