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Literature summary for 3.2.1.73 extracted from
- Purification, characterization and NH2-terminal sequencing of an endo-(1->3,1->4)-beta-glucanase from rice (Oryza sativa L.) bran (1998), Plant Sci., 134, 3-10.
No PubMed abstract available
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
1 * 31000, SDS-PAGE | Oryza sativa |
| 32000 | - |
gel filtration | Oryza sativa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | - |
L. cv. Kirara 397 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oryza sativa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 387 | - |
- |
Oryza sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-D-glucan + H2O | beta-D-glucan from barley | Oryza sativa | additional information | oligosaccharides are formed as the major products, no glucose | ? |  |
| beta-D-glucan + H2O | beta-D-glucan from oat | Oryza sativa | additional information | oligosaccharides are formed as the major products, no glucose | ? | |
| lichenin + H2O | lichenin from Cetralia islandica and from Usnea barbata | Oryza sativa | additional information | - |
? | |
| additional information | no hydrolysis of laminarin | Oryza sativa | ? | - |
? | |
| additional information | no hydrolysis of carboxymethylcellulose | Oryza sativa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 31000, SDS-PAGE | Oryza sativa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
10 min, stable | Oryza sativa |
| 50 | - |
10 min, about 25% loss of activity | Oryza sativa |
| 60 | - |
10 min, complete inactivation | Oryza sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Oryza sativa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6 | pH 3.0: about 50% of maximal activity, pH 6.0: about 40% of maximal activity | Oryza sativa |
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