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Literature summary for 3.2.1.72 extracted from

  • Umemoto, Y.; Onishi, R.; Araki, T.
    Cloning of a novel gene encoding beta -1,3-xylosidase from a marine bacterium, Vibrio sp. strain XY-214, and characterization of the gene product (2008), Appl. Environ. Microbiol., 74, 305-308.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.72

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Vibrio sp.

Inhibitors

Inhibitors Comment Organism Structure
Ag+ more than 90% inhibition Vibrio sp.
Cu2+ more than 90% inhibition Vibrio sp.
Hg2+ more than 90% inhibition Vibrio sp.
Mn2+ more than 90% inhibition Vibrio sp.
p-chloromercuribenzoic acid more than 90% inhibition Vibrio sp.
Pb2+ more than 90% inhibition Vibrio sp.
Zn2+ more than 90% inhibition Vibrio sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.244
-
 p-nitrophenyl-beta-D-xylopyranoside
-
 Vibrio sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 SDS-PAGE Vibrio sp.

Organism

Organism UniProt Comment Textmining
Vibrio sp. A9ZND1
-
-
Vibrio sp. XY-214 A9ZND1
-
-

Purification (Commentary)

Purification (Comment) Organism
wild type by DEAE-Toyopearl 650M and MonoQ 5/50 column chromatography, recombinant protein by HiTrap chelating HP column chromatography Vibrio sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta -1,4-xylotetraose low activity Vibrio sp. D-xylose
-
 ?
beta -1,4-xylotetraose low activity Vibrio sp. XY-214 D-xylose
-
 ?
beta-1,3-xylobiose + H2O complete hydrolysis Vibrio sp. D-xylose
-
 ?
beta-1,3-xylobiose + H2O complete hydrolysis Vibrio sp. XY-214 D-xylose
-
 ?
beta-1,3-xylotetraose + H2O complete hydrolysis Vibrio sp. D-xylose
-
 ?
beta-1,3-xylotetraose + H2O complete hydrolysis Vibrio sp. XY-214 D-xylose
-
 ?
beta-1,3-xylotriose + H2O complete hydrolysis Vibrio sp. D-xylose
-
 ?
beta-1,3-xylotriose + H2O complete hydrolysis Vibrio sp. XY-214 D-xylose
-
 ?
beta-1,4-xylobiose low activity Vibrio sp. D-xylose
-
 ?
beta-1,4-xylotriose low activity Vibrio sp. D-xylose
-
 ?
beta-1,4-xylotriose low activity Vibrio sp. XY-214 D-xylose
-
 ?
p-nitrophenyl-beta-D-xylopyranose + H2O no activity on laminarioligosaccharides, beta-1,3-linked D-glucose units, beta-1,3-xylan, beta-1,4-xylan, carboxymethyl cellulose, laminaran, p-nitrophenyl-beta-D-xylopyranoside, p-nitrophenyl-beta-D-mannopyranoside, p-nitrophenyl-beta-D-mannopyranoside, p-nitrophenyl-beta-Dgalactopyranoside, p-nitrophenyl-beta-D-glucopyranoside, p-nitrophenyl-beta-D-glucopyranoside, p-nitrophenyl-beta-L-fucopyranoside, p-nitrophenyl-beta-L-arabinofuranoside, p-nitrophenyl-beta-L-arabinopyranoside, p-nitrophenyl-beta-D-glucuronide, and p-nitrophenyl-beta-D-cellobioside Vibrio sp. p-nitrophenol + D-xylopyranose
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 60000 gel filtration chromatography on a Superdex 200 HR 10/30 column Vibrio sp.

Synonyms

Synonyms Comment Organism
beta-1,3-xylosidase A intracellular Vibrio sp.
xloA
-
 Vibrio sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Vibrio sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 with 2 mM p-nitrophenyl-beta-D-xylopyranoside at 37°C Vibrio sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 retaining more than 80% of the original activity after preincubation in the buffer at various pHs at 4°C for 12 h Vibrio sp.