Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) CodonPlus-RIL cells | Streptococcus mutans serotype c |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method. Mutant E236Q glucosyl-enzyme intermediate are obtained by co-crystallization with 5 mM alpha-D-glucosyl fluoride at 100 mM Tris-HCl (pH 7.5), 200 mM calcium chloride and 18% (w/v) polyethylene glycol (PEG) 6000. The ligand-free E236Q crystals are grown by using 100 mM sodium 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (pH 7.4), 200 mM calcium chloride and 18% (w/v) PEG 6000 | Streptococcus mutans serotype c |
Protein Variants | Comment | Organism |
---|---|---|
F262A | the mutant enzyme has a stronger preference for transglucosylation than that of the wild type enzyme | Streptococcus mutans serotype c |
F262W | the mutant enzyme has a weaker preference for transglucosylation than that of the wild type enzyme | Streptococcus mutans serotype c |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Streptococcus mutans serotype c | the enzyme catalyzes both the hydrolysis of substrates such as isomaltooligosaccharides and subsequent transglucosylation to form alpha-(1->6)-glucosidic linkage at the substrate non-reducing ends | ? | - |
? | |
additional information | Streptococcus mutans serotype c ATCC 700610 | the enzyme catalyzes both the hydrolysis of substrates such as isomaltooligosaccharides and subsequent transglucosylation to form alpha-(1->6)-glucosidic linkage at the substrate non-reducing ends | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus mutans serotype c | Q99040 | - |
- |
Streptococcus mutans serotype c ATCC 700610 | Q99040 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-chelating column chromatography and Superdex 200 gel filtration | Streptococcus mutans serotype c |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Streptococcus mutans serotype c | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Streptococcus mutans serotype c ATCC 700610 | 4-nitrophenol + D-glucopyranose | - |
? | |
isomaltose + H2O | - |
Streptococcus mutans serotype c | 2 D-glucose | - |
? | |
isomaltose + H2O | - |
Streptococcus mutans serotype c ATCC 700610 | 2 D-glucose | - |
? | |
additional information | the enzyme catalyzes both the hydrolysis of substrates such as isomaltooligosaccharides and subsequent transglucosylation to form alpha-(1->6)-glucosidic linkage at the substrate non-reducing ends | Streptococcus mutans serotype c | ? | - |
? | |
additional information | the enzyme catalyzes both the hydrolysis of substrates such as isomaltooligosaccharides and subsequent transglucosylation to form alpha-(1->6)-glucosidic linkage at the substrate non-reducing ends | Streptococcus mutans serotype c ATCC 700610 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dextran glucosidase | - |
Streptococcus mutans serotype c |