Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Arthrobacter globiformis |
Protein Variants | Comment | Organism |
---|---|---|
E458Q | mutant enzyme shows 0.0005% of the wild-type activity | Arthrobacter globiformis |
E656Q | mutant enzyme shows 0.0003% of the wild-type activity | Arthrobacter globiformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | KM-value for dextran is 0.031 mg/ml | Arthrobacter globiformis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
109135 | - |
x * 109135, calculated from sequence | Arthrobacter globiformis |
120000 | - |
x * 120000, SDS-PAGE | Arthrobacter globiformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter globiformis | - |
- |
- |
Arthrobacter globiformis I42 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type enzyme and mutant enzymes E458Q and E656Q | Arthrobacter globiformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dextran + H2O | - |
Arthrobacter globiformis | glucose | - |
? | |
dextran + H2O | - |
Arthrobacter globiformis I42 | glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 120000, SDS-PAGE | Arthrobacter globiformis |
? | x * 109135, calculated from sequence | Arthrobacter globiformis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Arthrobacter globiformis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
10 min, less than 20% loss of activity | Arthrobacter globiformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Arthrobacter globiformis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.4 | more than 80% of maximal activity betwenn pH 5.5 and pH 7.4 | Arthrobacter globiformis |