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Literature summary for 3.2.1.70 extracted from

  • MMorimoto, N.; Yasukawa, Y.; Watanabe, K.; Unno, T.; Ito, H.; Matsui, H.
    Cloning and heterologous expression of a glucodextranase gene from Arthrobacter globiformis I42, and experimental evidence for the catalytic diad of the recombinant enzyme (2004), J. Biosci. Bioeng., 97, 127-130.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arthrobacter globiformis

Protein Variants

Protein Variants Comment Organism
E458Q mutant enzyme shows 0.0005% of the wild-type activity Arthrobacter globiformis
E656Q mutant enzyme shows 0.0003% of the wild-type activity Arthrobacter globiformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information KM-value for dextran is 0.031 mg/ml Arthrobacter globiformis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
109135
-
x * 109135, calculated from sequence Arthrobacter globiformis
120000
-
x * 120000, SDS-PAGE Arthrobacter globiformis

Organism

Organism UniProt Comment Textmining
Arthrobacter globiformis
-
-
-
Arthrobacter globiformis I42
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type enzyme and mutant enzymes E458Q and E656Q Arthrobacter globiformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dextran + H2O
-
Arthrobacter globiformis glucose
-
?
dextran + H2O
-
Arthrobacter globiformis I42 glucose
-
?

Subunits

Subunits Comment Organism
? x * 120000, SDS-PAGE Arthrobacter globiformis
? x * 109135, calculated from sequence Arthrobacter globiformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Arthrobacter globiformis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
10 min, less than 20% loss of activity Arthrobacter globiformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Arthrobacter globiformis

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.4 more than 80% of maximal activity betwenn pH 5.5 and pH 7.4 Arthrobacter globiformis