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Literature summary for 3.2.1.7 extracted from

  • Karboune, S.; Appanah, N.; Khodaei, N.; Tian, F.
    Enzymatic synthesis of fructooligosaccharides from sucrose by endo-inulinase-catalyzed transfructosylation reaction in biphasic systems (2018), Process Biochem., 69, 82-91 .
No PubMed abstract available
Search for more literature for 3.2.1.7

Application

Application Comment Organism
synthesis enzymatic synthesis of fructooligosaccharides (FOS) from sucrose by endo-inulinase-catalyzed transfructosylation reaction in biphasic systems, production of FOS from sucrose by commercial inulinase from Aspergillus niger Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger O74641
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Purification (Commentary)

Purification (Comment) Organism
the commercial preparation of endoinulase is further purified 8.2fold by anion exchange chromatography to exclude any exoinulase activity Aspergillus niger

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
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 Aspergillus niger
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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 specific activity of the crude commercial inulinase enzyme is estimated to be 1.2596 mmol/mg protein/min, while that of the purified endo-inulinase enzyme is found to be 10.372 mmol/mg protein/min Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzymatic synthesis of fructooligosaccharides (FOS) from sucrose by endo-inulinase-catalyzed transfructosylation reaction of sucrose in biphasic systems, production of FOS from sucrose by commercial inulinase from Aspergillus niger, modeling, overview. With the use of maple syrup 66°Bx-based biphasic medium, kestose, nystose, and fructosyl-nystose are all produced during the first 16 h, with a relative proportion of 78.79%, 20.34%, and 0.86%, respectively Aspergillus niger ?
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 ?

Synonyms

Synonyms Comment Organism
endo-inulinase
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 Aspergillus niger
inu1
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 Aspergillus niger

General Information

General Information Comment Organism
additional information reaction selectivity of transfructosylation vs. hydrolysis of endo-inulinase from Aspergillus niger in selected biphasic media, overview Aspergillus niger