Cloned (Comment) | Organism |
---|---|
gene inuA, recombinant overexpresssion in Yarrowia lipolytica strain Enop56 (engineered from strain Po1h), during fermentation with an inulin concentration of 600 g/l in a 10-l bioreactor, the inulinase activity, fructooligosaccharides (FOS) titer, yield, and productivity reach 551.6 U/ml, 546.6 g/l, 0.91 gFOS/g Inulin, and 15.18 g/l/h, respectively | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | O74641 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | deglycosylation of the recombinant enzyme by endoglycosidase Endo H | Aspergillus niger |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Yarrowia lipolytica strain ENop56 by dialysis and anion exchange chromatography, the enzyme is then deglycosylated by endoglycosidase Endo H, followed by gel filtration | Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1206.63 | - |
purified recombinant enzyme, pH 4.5, 60°C | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inulin + H2O | - |
Aspergillus niger | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56510, deglycosylated recombinant enzyme, SDS-PAGE | Aspergillus niger |
Synonyms | Comment | Organism |
---|---|---|
endo-inulinase | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
recombinant enzyme | Aspergillus niger |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 70 | maximal activity at 60°C, 70% activity at 35°C, 80% at 50°C and 70°C, profile overview | Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
purified recombinant enzyme, 120 min, 90% activity remaining | Aspergillus niger |
60 | - |
purified recombinant enzyme, 120 min, 60% activity remaining | Aspergillus niger |
70 | - |
purified recombinant enzyme, 120 min, 10% activity remaining | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
recombinant enzyme | Aspergillus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 7 | over 70% of maximal activity within this range, profile overview | Aspergillus niger |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | 8 | purified recombinant enzyme, 120 min, over 50% activity remaining, completely stable at pH 4.5, profile overview | Aspergillus niger |