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Literature summary for 3.2.1.7 extracted from
- Purification and characterization of an endoinulinase from Xanthomonas oryzae No.5 (2002), Process Biochem., 37, 1325-1331.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| nutrition | production of oligosaccharides, which are soluble dietary fibers and/or a functional sweeteners | Xanthomonas oryzae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| inulin | concentrations above 125 g/l | Xanthomonas oryzae |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | inulin, 16.7 g/l, pH 7.5, at 50ºC | Xanthomonas oryzae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Xanthomonas oryzae | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 139000 | - |
SDS-PAGE | Xanthomonas oryzae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| inulin + H2O | Xanthomonas oryzae | - |
inulooligosaccharides | - |
? | |
| inulin + H2O | Xanthomonas oryzae No.5 | - |
inulooligosaccharides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Xanthomonas oryzae | - |
No.5 | - |
| Xanthomonas oryzae No.5 | - |
No.5 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no modification | - |
Xanthomonas oryzae |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by ammonium sulfate precipitation, DEAE-Sephacel column and phenyl-Sepharose column, 28.7fold | Xanthomonas oryzae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Xanthomonas oryzae | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1407 | - |
pH 6.0, at 45ºC | Xanthomonas oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| inulin + H2O | - |
Xanthomonas oryzae | inulooligosaccharides | - |
? | |
| inulin + H2O | - |
Xanthomonas oryzae No.5 | inulooligosaccharides | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endoinulinase | - |
Xanthomonas oryzae |
| fructofuranosyl hydrolase | - |
Xanthomonas oryzae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
pH 6.0 | Xanthomonas oryzae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
pH 6.0, up to 45ºC | Xanthomonas oryzae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
at 45ºC | Xanthomonas oryzae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | at 20ºC | Xanthomonas oryzae |
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