Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Lederbergia lentus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | Lederbergia lentus | the enzyme has the ability to attack side chains composed of 1-3 glucose residues | amylose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lederbergia lentus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Ni-NTA column chromatography, and Superdex 200 gel filtration | Lederbergia lentus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycogen + H2O | - |
Lederbergia lentus | ? | - |
? | |
maize amylopectin + H2O | isoamylase hydrolyses both inner and outer branching linkages of amylopectin simultaneously | Lederbergia lentus | ? | - |
? | |
maize starch + H2O | - |
Lederbergia lentus | ? | - |
? | |
additional information | no activity with amylose | Lederbergia lentus | ? | - |
? | |
potato starch + H2O | - |
Lederbergia lentus | ? | - |
? | |
soluble starch + H2O | - |
Lederbergia lentus | ? | - |
? | |
starch + H2O | the enzyme has the ability to attack side chains composed of 1-3 glucose residues | Lederbergia lentus | amylose | - |
? | |
waxy rice starch + H2O | - |
Lederbergia lentus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 108000, SDS-PAGE | Lederbergia lentus |
Synonyms | Comment | Organism |
---|---|---|
glycogen-6-glucanohydrolase | - |
Lederbergia lentus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Lederbergia lentus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Lederbergia lentus |