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Literature summary for 3.2.1.68 extracted from
- Differential chain-length specificities of two isoamylase-type starch-debranching enzymes from developing seeds of kidney bean (2007), Biosci. Biotechnol. Biochem., 71, 2308-2312.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
1 * 60000, SDS-PAGE | Phaseolus vulgaris |
| 80000 | - |
gel permeation chromatography | Phaseolus vulgaris |
| 87000 | - |
heterotetramer with PvISA1, 2 * 87000 PyISA1 and 2 * 93000 PvISA2, SDS-PAGE | Phaseolus vulgaris |
| 87000 | - |
heterotetramer with PvISA2, 2 * 87000 PvISA1 and 2 * 93000 PvISA2, SDS-PAGE | Phaseolus vulgaris |
| 93000 | - |
heterotetramer with PvISA1, 2 * 87000 PyISA1 and 2 * 93000 PvISA2, SDS-PAGE | Phaseolus vulgaris |
| 93000 | - |
heterotetramer with PvISA2, 2 * 87000 PvISA1 and 2 * 93000 PvISA2, SDS-PAGE | Phaseolus vulgaris |
| 370000 | - |
gel permeation chromatography | Phaseolus vulgaris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaseolus vulgaris | A4PIS8 | kidney bean | - |
| Phaseolus vulgaris | A4PIS9 | kidney bean | - |
| Phaseolus vulgaris | A4PIT0 | kidney bean | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Q sepharose column chromatography | Phaseolus vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Phaseolus vulgaris | - |
| seed | - |
Phaseolus vulgaris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.77 | - |
pullulan as substrate | Phaseolus vulgaris |
| 0.95 | - |
pullulan as substrate | Phaseolus vulgaris |
| 3.51 | - |
amylopectin as substrate | Phaseolus vulgaris |
| 6.58 | - |
beta limit dextrin as substrate | Phaseolus vulgaris |
| 8.95 | - |
gylcogen as substrate | Phaseolus vulgaris |
| 14.98 | - |
amylopectin as substrate | Phaseolus vulgaris |
| 17.16 | - |
gylcogen as substrate | Phaseolus vulgaris |
| 29.05 | - |
beta limit dextrin as substrate | Phaseolus vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Phaseolus vulgaris | linear maltooligosaccharides | - |
? |  |
| beta limit dextrin + H2O | - |
Phaseolus vulgaris | linear maltooligosaccharides | - |
? | |
| glycogen + H2O | - |
Phaseolus vulgaris | linear maltooligosaccharides | - |
? | |
| pullulan + H2O | - |
Phaseolus vulgaris | linear maltooligosaccharides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 60000, SDS-PAGE | Phaseolus vulgaris |
| tetramer | heterotetramer with PvISA1, 2 * 87000 PyISA1 and 2 * 93000 PvISA2, SDS-PAGE | Phaseolus vulgaris |
| tetramer | heterotetramer with PvISA2, 2 * 87000 PvISA1 and 2 * 93000 PvISA2, SDS-PAGE | Phaseolus vulgaris |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glycogen alpha-1,6-glucanohydrolase | - |
Phaseolus vulgaris |
| isoamylase | - |
Phaseolus vulgaris |
| PvISA1 | - |
Phaseolus vulgaris |
| PvISA2 | - |
Phaseolus vulgaris |
| PvISA3 | - |
Phaseolus vulgaris |
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Release 2023.1 (January 2023)
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