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Literature summary for 3.2.1.68 extracted from
- Cloning and characterization of an intracellular isoamylase gene from Pectobacterium chrysanthemi PY35 (2001), Biochem. Biophys. Res. Commun., 287, 348-354.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Dickeya chrysanthemi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ammonium sulfate | 0.5 M, 30% residual activity | Dickeya chrysanthemi |  |
| CuCl2 | 5 mM, 30% residual activity | Dickeya chrysanthemi | |
| EDTA | 5 mM, 80% residual activity | Dickeya chrysanthemi | |
| MgCl2 | 5 mM, 70% residual activity | Dickeya chrysanthemi | |
| MnCl2 | 5 mM, 80% residual activity | Dickeya chrysanthemi | |
| sodium dodecylsulfate | 1 mM, no residual activity | Dickeya chrysanthemi | |
| ZnCl2 | 5 mM, 20% residual activity | Dickeya chrysanthemi | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | 5 mM, 120% of activity | Dickeya chrysanthemi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 74000 | - |
x * 74100, deduced from gene sequence, x * 74000, SDS-PAGE | Dickeya chrysanthemi |
| 74100 | - |
x * 74100, deduced from gene sequence, x * 74000, SDS-PAGE | Dickeya chrysanthemi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dickeya chrysanthemi | - |
PY35 | - |
| Dickeya chrysanthemi PY35 | - |
PY35 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Dickeya chrysanthemi | ? | hydrolysis of alpha-1,6-linkages | ? | |
| amylopectin + H2O | - |
Dickeya chrysanthemi PY35 | ? | hydrolysis of alpha-1,6-linkages | ? | |
| glycogen + H2O | 80% activity compared to amylopectin | Dickeya chrysanthemi | ? | - |
? | |
| glycogen + H2O | 80% activity compared to amylopectin | Dickeya chrysanthemi PY35 | ? | - |
? | |
| additional information | no substrate: dextran, amylose | Dickeya chrysanthemi | ? | - |
? | |
| additional information | no substrate: dextran, amylose | Dickeya chrysanthemi PY35 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 74100, deduced from gene sequence, x * 74000, SDS-PAGE | Dickeya chrysanthemi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Dickeya chrysanthemi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Dickeya chrysanthemi |
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