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Literature summary for 3.2.1.68 extracted from
- Molecular weight of the undegraded polypeptide chain of Pseudomonas amyloderamosa isoamylase (1980), Biochim. Biophys. Acta, 611, 390-393.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Pseudomonas amyloderamosa |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
2 * 46000, sedimentation equilibrium | Pseudomonas amyloderamosa |
| 52000 | - |
2 * 52000, gel filtration | Pseudomonas amyloderamosa |
| 86000 | 94000 | 86000, undegraded polypeptide chain, SDS-PAGE | Pseudomonas amyloderamosa |
| 88000 | - |
calculated by comparison with standard proteins | Pseudomonas amyloderamosa |
| 90000 | - |
undegraded polypeptide chain | Pseudomonas amyloderamosa |
| 94000 | - |
native enzyme, centrifugation analysis | Pseudomonas amyloderamosa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | Pseudomonas amyloderamosa | - |
? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas amyloderamosa | - |
strain KlC, revertant strain of Kl | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | or the beta-limit dextrins, cleaves the branching points completely in vitro | Pseudomonas amyloderamosa | maltose + maltooligosaccharides | - |
? | |
| glycogen + H2O | cleaves the branching points completely in vitro | Pseudomonas amyloderamosa | maltose + maltooligosaccharides | - |
? | |
| glycogen + H2O | or the beta-limit dextrins | Pseudomonas amyloderamosa | maltose + maltooligosaccharides | - |
? | |
| starch + H2O | - |
Pseudomonas amyloderamosa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 46000, sedimentation equilibrium | Pseudomonas amyloderamosa |
| dimer | 2 * 52000, gel filtration | Pseudomonas amyloderamosa |
| dimer | dimers probably obtained by partial proteolytic degradation | Pseudomonas amyloderamosa |
| monomer | gel electrophoresis | Pseudomonas amyloderamosa |
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