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Literature summary for 3.2.1.68 extracted from

  • Ishizaki, Y.; Taniguchi, H.; Maruyama, Y.; Nakamura, M.
    Debranching enzymes of potato tubers (Solanum tuberosum L.). I. Purification and some properties of potato isoamylase (1983), Agric. Biol. Chem., 47, 771-779.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 reagent in the preincubation mixture at concentrations 0.05-1% activates the enzyme by 45-72% Solanum tuberosum

Inhibitors

Inhibitors Comment Organism Structure
p-chloromercuribenzoate reversible inactivation Solanum tuberosum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
83000
-
1 * 83000 + 1 * 95000 Solanum tuberosum
95000
-
1 * 83000 + 1 * 95000 Solanum tuberosum
178000
-
native enzyme Solanum tuberosum
520000
-
gel filtration Solanum tuberosum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Solanum tuberosum
-
?
-
?

Organism

Organism UniProt Comment Textmining
Solanum tuberosum
-
potato, L. cul. var. "Danshaku"
-

Purification (Commentary)

Purification (Comment) Organism
-
Solanum tuberosum

Source Tissue

Source Tissue Comment Organism Textmining
tuber
-
Solanum tuberosum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8
-
-
Solanum tuberosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O or the beta-limit dextrins, cleaves the branching points completely in vitro Solanum tuberosum maltose + maltooligosaccharides hydrolysis of beta-limit dextrins forming maltotriose as main reaction product ?
amylose + H2O short chain Solanum tuberosum ?
-
?
glycogen + H2O cleaves the branching points completely in vitro Solanum tuberosum maltose + maltooligosaccharides
-
?
glycogen + H2O or the beta-limit dextrins Solanum tuberosum maltose + maltooligosaccharides
-
?
maltopentaose + H2O sugar immobilized on Sepharose 4B Solanum tuberosum ?
-
?
additional information direct debranching enzyme, attacks unmodified glycogen and/or amylopectin with hydrolysis of the 1,6-bond, smallest substrate is not known Solanum tuberosum ?
-
?
additional information distinguished from alpha-dextrin endo-1,6-alpha-glucosidase, EC 3.2.1.41, by the inability of isoamylase to attack pullulan, and by limited action on alpha-limit dextrins, action on glycogen however is complete in contrast to limited action by alpha-dextrin glucanohydrolase, 1,6-linkage hydrolysed only at branch point Solanum tuberosum ?
-
?
phythoglycogen + H2O highly branched polysaccharide, complete hydrolysis of the alpha-1,6-glucosidic bonds Solanum tuberosum ?
-
?
starch + H2O
-
Solanum tuberosum ?
-
?
starch + H2O soluble starch, immobilized on Sepharose 4B Solanum tuberosum ?
-
?

Subunits

Subunits Comment Organism
dimer 1 * 83000 + 1 * 95000 Solanum tuberosum

Synonyms

Synonyms Comment Organism
potato isoamylase
-
Solanum tuberosum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60
-
not active above, amylopectin Solanum tuberosum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
-
Solanum tuberosum
50
-
amylopectin, incubated in the absence of substrate enzyme stable only up to 45°C Solanum tuberosum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6 amylopectin Solanum tuberosum

pH Range

pH Minimum pH Maximum Comment Organism
4 8 not active below, amylopectin Solanum tuberosum