Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Rhodothermus marinus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
digalacturonic acid | substrate inhibition | Rhodothermus marinus | |
galacturonic acid | product inhibition | Rhodothermus marinus | |
polygalacturonic acid | substrate inhibition | Rhodothermus marinus | |
trigalacturonic acid | substrate inhibition | Rhodothermus marinus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000673 | - |
polygalacturonic acid | at pH 7.0 and 25°C | Rhodothermus marinus | |
0.00241 | - |
trigalacturonic acid | at pH 7.0 and 25°C | Rhodothermus marinus | |
0.012 | - |
digalacturonic acid | at pH 7.0 and 25°C | Rhodothermus marinus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
citrus pectin + H2O | Rhodothermus marinus | - |
galacturonic acid | - |
? | |
citrus pectin + H2O | Rhodothermus marinus ATCC 43812 | - |
galacturonic acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodothermus marinus | D0MEP1 | - |
- |
Rhodothermus marinus ATCC 43812 | D0MEP1 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 200 gel filtration | Rhodothermus marinus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
citrus pectin + H2O | - |
Rhodothermus marinus | galacturonic acid | - |
? | |
citrus pectin + H2O | - |
Rhodothermus marinus ATCC 43812 | galacturonic acid | - |
? | |
digalacturonic acid + H2O | - |
Rhodothermus marinus | 2 galacturonic acid | - |
? | |
digalacturonic acid + H2O | - |
Rhodothermus marinus ATCC 43812 | 2 galacturonic acid | - |
? | |
polygalacturonic acid + H2O | - |
Rhodothermus marinus | galacturonic acid | - |
? | |
polygalacturonic acid + H2O | - |
Rhodothermus marinus ATCC 43812 | galacturonic acid | - |
? | |
trigalacturonic acid + H2O | - |
Rhodothermus marinus | digalacturonic acid + galacturonic acid | - |
? | |
trigalacturonic acid + H2O | - |
Rhodothermus marinus ATCC 43812 | digalacturonic acid + galacturonic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
exopolygalacturonanase | - |
Rhodothermus marinus |
GH28 | - |
Rhodothermus marinus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
93.9 | - |
50% of the initial enzyme activity remains after 1 h incubation at 93.9°C | Rhodothermus marinus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.02 | - |
polygalacturonic acid | at pH 7.0 and 25°C | Rhodothermus marinus | |
11.7 | - |
digalacturonic acid | at pH 7.0 and 25°C | Rhodothermus marinus | |
21.9 | - |
trigalacturonic acid | at pH 7.0 and 25°C | Rhodothermus marinus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Rhodothermus marinus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000128 | - |
galacturonic acid | at pH 7.0 and 25°C | Rhodothermus marinus | |
0.071 | - |
polygalacturonic acid | at pH 7.0 and 25°C | Rhodothermus marinus | |
0.074 | - |
trigalacturonic acid | at pH 7.0 and 25°C | Rhodothermus marinus | |
0.137 | - |
digalacturonic acid | at pH 7.0 and 25°C | Rhodothermus marinus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
968 | - |
digalacturonic acid | at pH 7.0 and 25°C | Rhodothermus marinus | |
8950 | - |
polygalacturonic acid | at pH 7.0 and 25°C | Rhodothermus marinus | |
9080 | - |
trigalacturonic acid | at pH 7.0 and 25°C | Rhodothermus marinus |