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Literature summary for 3.2.1.67 extracted from
- Production, purification and biochemical characterization of an exo-polygalacturonase from Aspergillus niger MTCC 478 suitable for clarification of orange juice (2017), 3 Biotech, 7, 122 .
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | the enzyme is suitable for clarification of orange juice | Aspergillus niger |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 14.4% residual activity at 1 mM | Aspergillus niger |  |
| Ca2+ | 34% residual activity at 1 mM | Aspergillus niger | |
| Co2+ | 80% residual activity at 1 mM | Aspergillus niger | |
| EDTA | 61% residual activity at 1 mM | Aspergillus niger | |
| K+ | 36% residual activity at 1 mM | Aspergillus niger | |
| additional information | not inhibited by potassium permanganate | Aspergillus niger | |
| potassium ferrocyanide | 65% residual activity at 1 mM | Aspergillus niger | |
| Zn2+ | 38% residual activity at 1 mM | Aspergillus niger | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 148% activity at 1 mM | Aspergillus niger | |
| additional information | not influenced by Hg2+, Na+ and Mn2+ | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
| Aspergillus niger MTCC 478 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| acetone precipitation and carboxymethyl cellulose column chromatography | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polygalacturonic acid + H2O | - |
Aspergillus niger | galacturonic acid | - |
? | |
| polygalacturonic acid + H2O | - |
Aspergillus niger MTCC 478 | galacturonic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 124000, SDS-PAGE | Aspergillus niger |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Exo-PG | - |
Aspergillus niger |
| exo-polygalacturonase | - |
Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Aspergillus niger |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | more than 60% activity between 30 and 70°C | Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 50 | the enzyme retains its maximum activity between 10 and 40°C for 30 min. The maximum stability is up to 40°C and the activity declines sharply above this temperature. Approximately 53% of the activity is retained at 50°C | Aspergillus niger |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 194 | - |
polygalacturonic acid | at pH 4.0 and 40°C | Aspergillus niger | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Aspergillus niger |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 11 | the enzyme is stable for pH values of 3.0-11.0 when exposed to buffers of various pH for 24 h | Aspergillus niger |
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