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Literature summary for 3.2.1.67 extracted from

  • Mertens, J.A.
    Kinetic properties of two Rhizopus exo-polygalacturonase enzymes hydrolyzing galacturonic acid oligomers using isothermal titration calorimetry (2013), Appl. Biochem. Biotechnol., 170, 2009-2020.
    View publication on PubMed
Search for more literature for 3.2.1.67

Protein Variants

Protein Variants Comment Organism
D210N isozyme RPG15 mutant, the mutant shows a 5000fold reduced specific activity relative to the wild-type, rendering the enzyme essentially inactive Rhizopus arrhizus

Inhibitors

Inhibitors Comment Organism Structure
D-galacturonate product inhibition Rhizopus arrhizus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information thermodynamic parameters of a series of galacturonic acid oligomers binding to isozyme RPG15, overview Rhizopus arrhizus
0.0092
-
 [(1->4)-alpha-D-galacturonide]3 isozyme RPG15, pH 4.0, 30°C Rhizopus arrhizus
0.016
-
 [(1->4)-alpha-D-galacturonide]2 isozyme RPG16, pH 4.0, 30°C Rhizopus arrhizus
0.055
-
 [(1->4)-alpha-D-galacturonide]2 isozyme RPG15, pH 4.0, 30°C Rhizopus arrhizus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[(1->4)-alpha-D-galacturonide]n + H2O Rhizopus arrhizus
-
 [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate
-
 ?

Organism

Organism UniProt Comment Textmining
Rhizopus arrhizus Q5W9U0 two isozymes RPG15 and RPG16
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[(1->4)-alpha-D-galacturonide]2 + H2O
-
 Rhizopus arrhizus 2 D-galacturonate
-
 ?
[(1->4)-alpha-D-galacturonide]3 + H2O
-
 Rhizopus arrhizus [(1->4)-alpha-D-galacturonide]2 + D-galacturonate
-
 ?
[(1->4)-alpha-D-galacturonide]n + H2O
-
 Rhizopus arrhizus [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate
-
 ?
[(1->4)-alpha-D-galacturonide]n + H2O the two Rhizopus oryzae exo-polygalacturonase isozymes act on galacturonic acid oligomers, isothermal titration calorimetry analysis, overview Rhizopus arrhizus [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate
-
 ?

Synonyms

Synonyms Comment Organism
Exo-PG
-
 Rhizopus arrhizus
exo-polygalacturonase
-
 Rhizopus arrhizus
RPG15
-
 Rhizopus arrhizus
RPG16
-
 Rhizopus arrhizus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Rhizopus arrhizus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.9
-
 [(1->4)-alpha-D-galacturonide]2 isozyme RPG16, pH 4.0, 30°C Rhizopus arrhizus
10.3
-
 [(1->4)-alpha-D-galacturonide]2 isozyme RPG15, pH 4.0, 30°C Rhizopus arrhizus
10.7
-
 [(1->4)-alpha-D-galacturonide]3 isozyme RPG15, pH 4.0, 30°C Rhizopus arrhizus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 assay at Rhizopus arrhizus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.501
-
 D-galacturonate isozyme RPG16, pH 4.0, 30°C Rhizopus arrhizus
0.886
-
 D-galacturonate isozyme RPG15, pH 4.0, 30°C Rhizopus arrhizus

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycosyl hydrolase 28 family, GH28 Rhizopus arrhizus