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Literature summary for 3.2.1.67 extracted from

  • Stratilova, E.; Dzurova, M.; Malovikova, A.; Omelkova, J.
    Oligogalacturonate hydrolase from carrot roots (2006), Z. Naturforsch. C, 60, 899-905.
    View publication on PubMed
Search for more literature for 3.2.1.67

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 (1,4-alpha-D-galacturonide)8 0.1 M acetate buffer pH 3.8, 30°C Daucus carota
0.029
-
 (1,4-alpha-D-galacturonide)7 0.1 M acetate buffer pH 3.8, 30°C Daucus carota
0.057
-
 (1,4-alpha-D-galacturonide)6 0.1 M acetate buffer pH 3.8, 30°C Daucus carota
0.077
-
 (1,4-alpha-D-galacturonide)5 0.1 M acetate buffer pH 3.8, 30°C Daucus carota
0.1
-
 (1,4-alpha-D-galacturonide)4 0.1 M acetate buffer pH 3.8, 30°C Daucus carota
0.19
-
 (1,4-alpha-D-galacturonide)3 0.1 M acetate buffer pH 3.8, 30°C Daucus carota
0.86
-
 (1,4-alpha-D-galacturonide)2 0.1 M acetate buffer pH 3.8, 30°C Daucus carota

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Daucus carota
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 gel filtration Daucus carota

Organism

Organism UniProt Comment Textmining
Daucus carota
-
 cv. Tiptop
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N-glycosidase F cleavage reduced the apparent molecular weight on SDS-PAGE from 54 kDa to 31.5 kDa Daucus carota

Purification (Commentary)

Purification (Comment) Organism
3 chromatography steps Daucus carota

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Daucus carota
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1,4-alpha-D-galacturonide)2 + H2O
-
 Daucus carota D-galacturonic acid
-
 ?
(1,4-alpha-D-galacturonide)3 + H2O
-
 Daucus carota D-galacturonic acid
-
 ?
(1,4-alpha-D-galacturonide)4 + H2O
-
 Daucus carota D-galacturonic acid
-
 ?
(1,4-alpha-D-galacturonide)5 + H2O
-
 Daucus carota D-galacturonic acid
-
 ?
(1,4-alpha-D-galacturonide)6 + H2O
-
 Daucus carota D-galacturonic acid
-
 ?
(1,4-alpha-D-galacturonide)7 + H2O
-
 Daucus carota D-galacturonic acid
-
 ?
(1,4-alpha-D-galacturonide)8 + H2O
-
 Daucus carota D-galacturonic acid
-
 ?
pectic acid + H2O
-
 Daucus carota D-galacturonic acid
-
 ?

Synonyms

Synonyms Comment Organism
oligogalacturonate hydrolase
-
 Daucus carota

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Daucus carota

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 90% activity after 2 h at 60°C, rapid decrease of activity at 70°C Daucus carota

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.17
-
 (1,4-alpha-D-galacturonide)2 0.1 M acetate buffer pH 3.8, 30°C Daucus carota
0.31
-
 (1,4-alpha-D-galacturonide)8 0.1 M acetate buffer pH 3.8, 30°C Daucus carota
0.33
-
 (1,4-alpha-D-galacturonide)7 0.1 M acetate buffer pH 3.8, 30°C Daucus carota
0.44
-
 (1,4-alpha-D-galacturonide)3 0.1 M acetate buffer pH 3.8, 30°C Daucus carota
0.52
-
 (1,4-alpha-D-galacturonide)4 0.1 M acetate buffer pH 3.8, 30°C Daucus carota
0.57
-
 (1,4-alpha-D-galacturonide)5 0.1 M acetate buffer pH 3.8, 30°C Daucus carota
0.59
-
 (1,4-alpha-D-galacturonide)6 0.1 M acetate buffer pH 3.8, 30°C Daucus carota

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8
-
-
 Daucus carota

pI Value

Organism Comment pI Value Maximum pI Value
Daucus carota
-
-
 4.5