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Literature summary for 3.2.1.67 extracted from

  • Behere, A.; Satyanarayan, V.; Pawal-Desai, S.R.
    Separation and limited characterization of three polygalacturonases from Aspergillus niger (1993), Enzyme Microb. Technol., 15, 158-161.
No PubMed abstract available

Application

Application Comment Organism
nutrition enzymes degrading pectic substances to improve cloud stability of fruit and vegetable nectars, clarification of fruit juice and wine, production of baby food , commercial preparations of pectolytic enzymes for food processing industry Aspergillus niger
synthesis pectic enzymes used to generate protoplasts in cell cultures Aspergillus niger
synthesis as adjunct to cellulases and hemicellulases in cellulosic biomass treatment Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
pectic acid enzyme form PGII Aspergillus niger
0.24
-
pectic acid enzyme form PGI Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.8
-
enzyme form PGII Aspergillus niger
7.5
-
enzyme form PGI Aspergillus niger

Storage Stability

Storage Stability Organism
0-4°C, 3 months 90% activity retained, PGII 28°C 24 h 85% activity retained, PGI is unstable, cannot be stored Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pectic acid + H2O 2 exoforms of enzyme, PGI and PGII, prefer NaPPas substrate over pectin Aspergillus niger D-galacturonic acid + ?
-
?
pectic acid + H2O lytic cleavage of NaPP, degradation in vitro Aspergillus niger D-galacturonic acid + ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
all 3 forms of enzyme Aspergillus niger