Application | Comment | Organism |
---|---|---|
nutrition | enzymes degrading pectic substances to improve cloud stability of fruit and vegetable nectars, clarification of fruit juice and wine, production of baby food , commercial preparations of pectolytic enzymes for food processing industry | Aspergillus niger |
synthesis | pectic enzymes used to generate protoplasts in cell cultures | Aspergillus niger |
synthesis | as adjunct to cellulases and hemicellulases in cellulosic biomass treatment | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
pectic acid | enzyme form PGII | Aspergillus niger | |
0.24 | - |
pectic acid | enzyme form PGI | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.8 | - |
enzyme form PGII | Aspergillus niger |
7.5 | - |
enzyme form PGI | Aspergillus niger |
Storage Stability | Organism |
---|---|
0-4°C, 3 months 90% activity retained, PGII 28°C 24 h 85% activity retained, PGI is unstable, cannot be stored | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectic acid + H2O | 2 exoforms of enzyme, PGI and PGII, prefer NaPPas substrate over pectin | Aspergillus niger | D-galacturonic acid + ? | - |
? | |
pectic acid + H2O | lytic cleavage of NaPP, degradation in vitro | Aspergillus niger | D-galacturonic acid + ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
all 3 forms of enzyme | Aspergillus niger |