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Literature summary for 3.2.1.63 extracted from

  • Megson, Z.A.; Koerdt, A.; Schuster, H.; Ludwig, R.; Janesch, B.; Frey, A.; Naylor, K.; Wilson, I.B.; Stafford, G.P.; Messner, P.; Schaeffer, C.
    Characterization of an alpha-L-fucosidase from the periodontal pathogen Tannerella forsythia (2015), Virulence, 6, 282-292 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.63

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Tannerella forsythia

Inhibitors

Inhibitors Comment Organism Structure
deoxyfuconojirimycin complete inhibition at 0.0001 mM Tannerella forsythia
L-fucose complete inhibition at 0.25 mM Tannerella forsythia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.67
-
 4-nitrophenyl alpha-L-fucopyranoside at pH 9.0 and 22°C Tannerella forsythia

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Tannerella forsythia 16020
-

Organism

Organism UniProt Comment Textmining
Tannerella forsythia
-
-
-
Tannerella forsythia ATCC 43037
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA bead chromatography Tannerella forsythia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-fucosyllactose + H2O
-
 Tannerella forsythia L-fucose + lactose
-
 ?
2'-fucosyllactose + H2O
-
 Tannerella forsythia ATCC 43037 L-fucose + lactose
-
 ?
4-nitrophenyl alpha-L-fucopyranoside + H2O
-
 Tannerella forsythia 4-nitrophenol + L-fucopyranose
-
 ?
4-nitrophenyl alpha-L-fucopyranoside + H2O
-
 Tannerella forsythia ATCC 43037 4-nitrophenol + L-fucopyranose
-
 ?
alpha-L-Fuc-(1->6)-beta-D-GlcNAc + H2O the enzyme also possesses alpha(1,6) specificity on small unbranched substrates Tannerella forsythia ?
-
 ?
alpha-L-Fuc-(1->6)-beta-D-GlcNAc + H2O the enzyme also possesses alpha(1,6) specificity on small unbranched substrates Tannerella forsythia ATCC 43037 ?
-
 ?
bovine submaxillary mucin + H2O
-
 Tannerella forsythia L-fucose + ?
-
 ?
bovine submaxillary mucin + H2O
-
 Tannerella forsythia ATCC 43037 L-fucose + ?
-
 ?
additional information the alpha(1,3) and alpha(1,4) linkages are not cleaved on 3-fucosyllactose and the Lewis A trisaccharide, respectively. The enzyme is also inactive on A antigen tetraose and alpha-L-Fuc-(1->4)-beta-D-Gal Tannerella forsythia ?
-
 ?
additional information the alpha(1,3) and alpha(1,4) linkages are not cleaved on 3-fucosyllactose and the Lewis A trisaccharide, respectively. The enzyme is also inactive on A antigen tetraose and alpha-L-Fuc-(1->4)-beta-D-Gal Tannerella forsythia ATCC 43037 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 51000, SDS-PAGE Tannerella forsythia

Synonyms

Synonyms Comment Organism
alpha(1,2) fucosidase
-
 Tannerella forsythia
bfo_2737
-
 Tannerella forsythia
Fuc1
-
 Tannerella forsythia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Tannerella forsythia

General Information

General Information Comment Organism
physiological function the enzyme contributes to the virulence of Tannerella forsythia Tannerella forsythia