Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.63 extracted from

  • Fan, S.; Zhang, H.; Chen, X.; Lu, L.; Xu, L.; Xiao, M.
    Cloning, characterization, and production of three alpha-L-fucosidases from Clostridium perfringens ATCC 13124 (2016), J. Basic Microbiol., 56, 347-357 .
    View publication on PubMed
Search for more literature for 3.2.1.63

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Clostridium perfringens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.01
-
 alpha-L-Fuc-(1->2)-D-Gal at pH 7.0 and 37°C Clostridium perfringens

Organism

Organism UniProt Comment Textmining
Clostridium perfringens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-L-Fuc-(1->2)-D-Gal + H2O
-
 Clostridium perfringens L-fucose + ?
-
 ?
additional information the enzyme does not hydrolyze other fucosyloligosaccharides like Lewisa trisaccharide, Lewisx trisaccharide, 3-fucosyl-GlcNAc, 4-fucosyl-GlcNAc, 6-fucosyl-GlcNAc or 4-nitrophenyl-alpha-L-fucoside Clostridium perfringens ?
-
 ?
porcine gastric mucin + H2O
-
 Clostridium perfringens L-fucose + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 162000, SDS-PAGE Clostridium perfringens

Synonyms

Synonyms Comment Organism
1,2-alpha-L-fucosidase
-
 Clostridium perfringens
Afc3
-
 Clostridium perfringens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Clostridium perfringens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
55
-
 the enzyme maintains over 95% enzyme activity below 55°C Clostridium perfringens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15.4
-
 alpha-L-Fuc-(1->2)-D-Gal at pH 7.0 and 37°C Clostridium perfringens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Clostridium perfringens

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 11 the enzyme is stable at pH 4.0-11.0 Clostridium perfringens

Expression

Organism Comment Expression
Clostridium perfringens the transcription of the enzyme increases to 1.4fold in the presence of porcine gastric mucin up

General Information

General Information Comment Organism
metabolism the enzyme is involved in the degradation of intestinal fucosyl glycans by Clostridium perfringens ATCC 13124 Clostridium perfringens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.56
-
 alpha-L-Fuc-(1->2)-D-Gal at pH 7.0 and 37°C Clostridium perfringens