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Literature summary for 3.2.1.63 extracted from
- Glycosidases of Aspergillus niger. II. Purification and general properties of 1,2-alpha-L-fucosidase (1970), J. Biol. Chem., 245, 299-304.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.083 | - |
methyl 2-O-alpha-L-fucopyranosyl-beta-D-galactoside | - |
Aspergillus niger |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus niger |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | commercial preparation | Aspergillus niger | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.7 | - |
- |
Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-O-alpha-L-fucopyranosyl-D-galactose + H2O | - |
Aspergillus niger | L-fucose + galactose | - |
? | |
| alpha-Fuc-(1-2)-beta-Gal-(1-3)-beta-GlcNAc-(1-3)-beta-Gal-(1-4)-beta-Glc + H2O | i.e. lacto-N-fucopentaose I | Aspergillus niger | alpha-L-fucose + beta-Gal-(1-3)-beta-GlcNAc-(1-3)-beta-Gal-(1-4)-beta-Glc | - |
? | |
| methyl-2-alpha-L-fucopyranosyl-beta-D-galactoside + H2O | - |
Aspergillus niger | L-fucose + methyl beta-D-galactoside | - |
? | |
| additional information | highly specific for non-reducing terminal L-fucose residues linked to D-galactose residues by 1,2-alpha linkage | Aspergillus niger | ? | - |
? | |
| mucin + H2O | porcine and canine, submaxillary | Aspergillus niger | L-fucose + ? | - |
? | |
| O-alpha-L-fucose-(1-2)-O-beta-D-galactose-(1-4)-D-glucose + H2O | i.e. 2-O-alpha-L-fucosyllactose | Aspergillus niger | L-fucose + lactose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.8 | - |
- |
Aspergillus niger |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.4 | 7.5 | - |
Aspergillus niger |
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Release 2023.1 (January 2023)
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