BRENDA - Enzyme Database
show all sequences of 3.2.1.63

Glycosidases of Aspergillus niger. II. Purification and general properties of 1,2-alpha-L-fucosidase

Bahl, O.P.; J. Biol. Chem. 245, 299-304 (1970)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.083
-
methyl 2-O-alpha-L-fucopyranosyl-beta-D-galactoside
-
Aspergillus niger
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus niger
-
-
-
Purification (Commentary)
Commentary
Organism
-
Aspergillus niger
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
commercial preparation
Aspergillus niger
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.7
-
-
Aspergillus niger
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-O-alpha-L-fucopyranosyl-D-galactose + H2O
-
136749
Aspergillus niger
L-fucose + galactose
-
136749
Aspergillus niger
?
alpha-Fuc-(1-2)-beta-Gal-(1-3)-beta-GlcNAc-(1-3)-beta-Gal-(1-4)-beta-Glu + H2O
i.e. lacto-N-fucopentaose I
136749
Aspergillus niger
alpha-L-fucose + beta-Gal-(1-3)-beta-GlcNAc-(1-3)-beta-Gal-(1-4)-beta-Glu
-
136749
Aspergillus niger
?
methyl-2-alpha-L-fucopyranosyl-beta-D-galactoside + H2O
-
136749
Aspergillus niger
L-fucose + methyl beta-D-galactoside
-
136749
Aspergillus niger
?
additional information
highly specific for non-reducing terminal L-fucose residues linked to D-galactose residues by 1,2-alpha linkage
136749
Aspergillus niger
?
-
-
-
-
mucin + H2O
porcine and canine, submaxillary
136749
Aspergillus niger
L-fucose + ?
-
136749
Aspergillus niger
-
O-alpha-L-fucose-(1-2)-O-beta-D-galactose-(1-4)-D-glucose + H2O
i.e. 2-O-alpha-L-fucosyllactose
136749
Aspergillus niger
L-fucose + lactose
-
136749
Aspergillus niger
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Aspergillus niger
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.8
-
-
Aspergillus niger
pH Range
pH Minimum
pH Maximum
Commentary
Organism
2.4
7.5
-
Aspergillus niger
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.083
-
methyl 2-O-alpha-L-fucopyranosyl-beta-D-galactoside
-
Aspergillus niger
Purification (Commentary) (protein specific)
Commentary
Organism
-
Aspergillus niger
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
commercial preparation
Aspergillus niger
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.7
-
-
Aspergillus niger
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-O-alpha-L-fucopyranosyl-D-galactose + H2O
-
136749
Aspergillus niger
L-fucose + galactose
-
136749
Aspergillus niger
?
alpha-Fuc-(1-2)-beta-Gal-(1-3)-beta-GlcNAc-(1-3)-beta-Gal-(1-4)-beta-Glu + H2O
i.e. lacto-N-fucopentaose I
136749
Aspergillus niger
alpha-L-fucose + beta-Gal-(1-3)-beta-GlcNAc-(1-3)-beta-Gal-(1-4)-beta-Glu
-
136749
Aspergillus niger
?
methyl-2-alpha-L-fucopyranosyl-beta-D-galactoside + H2O
-
136749
Aspergillus niger
L-fucose + methyl beta-D-galactoside
-
136749
Aspergillus niger
?
additional information
highly specific for non-reducing terminal L-fucose residues linked to D-galactose residues by 1,2-alpha linkage
136749
Aspergillus niger
?
-
-
-
-
mucin + H2O
porcine and canine, submaxillary
136749
Aspergillus niger
L-fucose + ?
-
136749
Aspergillus niger
-
O-alpha-L-fucose-(1-2)-O-beta-D-galactose-(1-4)-D-glucose + H2O
i.e. 2-O-alpha-L-fucosyllactose
136749
Aspergillus niger
L-fucose + lactose
-
136749
Aspergillus niger
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Aspergillus niger
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.8
-
-
Aspergillus niger
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
2.4
7.5
-
Aspergillus niger
Other publictions for EC 3.2.1.63
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750713
Bishnoi
An F-type lectin domain direc ...
Streptosporangium roseum, Streptosporangium roseum DSM 43021
Glycobiology
28
860-875
2018
-
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1
-
2
-
19
1
-
3
-
-
-
2
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16
1
1
1
-
-
-
1
-
-
-
-
13
-
-
1
-
-
2
-
13
19
-
1
-
3
-
-
-
-
-
-
-
-
-
-
16
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
750985
Fan
Cloning, characterization, an ...
Clostridium perfringens
J. Basic Microbiol.
56
347-357
2016
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
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-
-
-
-
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3
1
1
1
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1
1
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1
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1
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1
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3
1
1
1
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1
1
-
1
-
1
1
1
1
1
1
752322
Megson
Characterization of an alpha- ...
Tannerella forsythia, Tannerella forsythia ATCC 43037
Virulence
6
282-292
2015
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1
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2
1
1
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1
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10
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1
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2
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1
1
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1
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10
1
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1
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1
1
-
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-
732150
Cao
Structure and substrate specif ...
Fusarium graminearum
J. Biol. Chem.
289
25624-25638
2014
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-
1
1
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1
-
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3
-
1
1
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3
1
1
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1
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1
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1
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1
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1
1
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3
1
1
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1
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-
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-
2
2
-
-
-
732382
Liu
Amide resonance in the catalys ...
Bifidobacterium bifidum
J. Phys. Chem. B
117
10080-10092
2013
-
-
-
-
1
-
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-
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1
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1
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1
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2
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1
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1
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2
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2
2
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713762
Wu
Structural basis of alpha-fuco ...
Thermotoga maritima
Angew. Chem.
49
337-340
2010
-
-
-
-
-
-
10
-
-
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-
1
-
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-
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-
9
-
-
-
-
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-
-
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-
10
9
-
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-
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-
-
708607
Ashida
Two distinct alpha-L-fucosidas ...
Bifidobacterium bifidum, no activity in Bifidobacterium longum
Glycobiology
19
1010-1017
2009
-
-
1
-
-
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-
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1
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8
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1
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1
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1
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1
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1
1
-
-
-
677707
Ruas-Madiedo
Mucin degradation by Bifidobac ...
Bifidobacterium bifidum
Appl. Environ. Microbiol.
74
1936-1940
2008
1
-
-
-
-
-
-
-
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1
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1
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1
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697936
Wada
1,2-alpha-l-Fucosynthase: a gl ...
Bifidobacterium bifidum
FEBS Lett.
582
3739-3743
2008
-
1
1
-
3
-
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-
-
-
-
-
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1
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1
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2
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1
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1
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2
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680783
Nagae
Structural basis of the cataly ...
Bifidobacterium bifidum
J. Biol. Chem.
282
18497-18509
2007
1
-
1
1
9
-
1
9
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2
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1
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2
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1
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1
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1
9
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1
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9
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1
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2
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665768
Katayama
Novel bifidobacterial glycosid ...
Bifidobacterium bifidum
J. Biosci. Bioeng.
99
457-465
2005
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-
1
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1
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3
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4
1
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1
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1
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1
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1
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4
1
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1
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1
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665770
Miura
Purification and characterizat ...
Bacillus cereus
J. Biosci. Bioeng.
99
629-635
2005
2
-
-
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-
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17
-
1
-
2
1
-
3
-
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1
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1
1
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5
1
1
1
2
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1
1
1
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1
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2
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17
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1
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2
1
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1
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1
1
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5
1
1
1
2
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1
1
1
1
-
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-
665375
Katayama
Molecular cloning and characte ...
Bifidobacterium bifidum
J. Bacteriol.
186
4885-4893
2004
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1
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1
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1
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3
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1
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1
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1
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1
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4
1
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1
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1
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136751
Larson
Degradation of human intestina ...
Bifidobacterium bifidum, Bifidobacterium bifidum VIII-210, Bifidobacterium longum subsp. infantis, Bifidobacterium longum subsp. infantis VIII-240, Ruminococcus gnavus, Ruminococcus torques
J. Biol. Chem.
263
10790-10798
1988
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6
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208732
Ogata-Arakawa
alpha-L-Fucosidases from almon ...
Prunus dulcis
Arch. Biochem. Biophys.
181
353-358
1977
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2
1
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1
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136753
Kochibe
Purification and properties of ...
Bacillus fulminans
J. Biochem.
74
1141-1149
1973
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5
5
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1
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4
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5
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5
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1
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4
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4
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136749
Bahl
Glycosidases of Aspergillus ni ...
Aspergillus niger
J. Biol. Chem.
245
299-304
1970
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1
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2
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6
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1
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6
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1
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1
1
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136752
Aminoff
Enzymes that destroy blood gro ...
Clostridium perfringens
J. Biol. Chem.
245
1659-1669
1970
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3
8
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1
1
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1
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1
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1
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3
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1
1
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3
-
8
-
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1
1
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1
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1
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3
-
-
-
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1
1
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