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Literature summary for 3.2.1.62 extracted from

  • Watanabe, T.; Tani, M.; Ishibashi, Y.; Endo, I.; Okino, N.; Ito, M.
    Ergosteryl-beta-glucosidase (Egh1) involved in sterylglucoside catabolism and vacuole formation in Saccharomyces cerevisiae (2015), Glycobiology, 25, 1079-1089 .
    View publication on PubMed
Search for more literature for 3.2.1.62

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae BY4741
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-conjugated Sepharose 6 column chromatography and Superdex 200 gel filtration Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumberifellyl beta-D-glucoside + H2O
-
 Saccharomyces cerevisiae 4-methylumbelliferone + beta-D-glucose
-
 ?
4-nitrophenyl alpha-D-galactoside + H2O very low activity Saccharomyces cerevisiae 4-nitrophenol + alpha-D-galactose
-
 ?
4-nitrophenyl alpha-D-glucoside + H2O very low activity Saccharomyces cerevisiae 4-nitrophenol + alpha-D-glucose
-
 ?
4-nitrophenyl beta-D-arabinopyranoside + H2O very low activity Saccharomyces cerevisiae 4-nitrophenol + beta-D-arabinopyranose
-
 ?
4-nitrophenyl beta-D-fucoside + H2O very low activity Saccharomyces cerevisiae 4-nitrophenol + beta-D-fucose
-
 ?
4-nitrophenyl beta-D-fucoside + H2O very low activity Saccharomyces cerevisiae BY4741 4-nitrophenol + beta-D-fucose
-
 ?
4-nitrophenyl beta-D-galactoside + H2O very low activity Saccharomyces cerevisiae 4-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-galactoside + H2O very low activity Saccharomyces cerevisiae BY4741 4-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-GlcNAc + H2O very low activity Saccharomyces cerevisiae 4-nitrophenol + beta-D-GlcNAc
-
 ?
4-nitrophenyl beta-D-glucoside + H2O
-
 Saccharomyces cerevisiae 4-nitrophenol + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-glucoside + H2O
-
 Saccharomyces cerevisiae BY4741 4-nitrophenol + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-xyloside + H2O very low activity Saccharomyces cerevisiae 4-nitrophenol + beta-D-xylose
-
 ?
C6-7-nitro-2,1,3-benzoxadiazole-GlcCer
-
 Saccharomyces cerevisiae C6-7-nitro-2,1,3-benzoxadiazole-ceramide + beta-D-glucose
-
 ?
cholesteryl beta-D-glucoside + H2O
-
 Saccharomyces cerevisiae cholesterol + beta-D-glucose
-
 ?
ergosteryl beta-D-glucoside + H2O
-
 Saccharomyces cerevisiae ergosterol + beta-D-glucose
-
 ?
glucosylceramide + H2O
-
 Saccharomyces cerevisiae beta-D-glucose + ceramide
-
 ?
glucosylceramide + H2O
-
 Saccharomyces cerevisiae BY4741 beta-D-glucose + ceramide
-
 ?
additional information no activity with C6-7-nitro-2,1,3-benzoxadiazole-sphingomyelin, 4-nitrophenyl alpha-D-fucoside, 4-nitrophenyl alpha-D-GalNAc, 4-nitrophenyl beta-D-GalNAc, 4-nitrophenyl alpha-D-GlcNAc, 4-nitrophenyl alpha-D-mannoside, 4-nitrophenyl beta-D-mannoside, alpha-D-xylose, 4-nitrophenyl alpha-D-arabinopyranoside, 4-nitrophenyl beta-D-arabinofuranoside, 4-nitrophenyl alpha-D-rhamnoside, 4-nitrophenyl alpha-D-sialoside, and 4-nitrophenyl beta-D-GlcUA Saccharomyces cerevisiae ?
-
 ?
additional information no activity with C6-7-nitro-2,1,3-benzoxadiazole-sphingomyelin, 4-nitrophenyl alpha-D-fucoside, 4-nitrophenyl alpha-D-GalNAc, 4-nitrophenyl beta-D-GalNAc, 4-nitrophenyl alpha-D-GlcNAc, 4-nitrophenyl alpha-D-mannoside, 4-nitrophenyl beta-D-mannoside, alpha-D-xylose, 4-nitrophenyl alpha-D-arabinopyranoside, 4-nitrophenyl beta-D-arabinofuranoside, 4-nitrophenyl alpha-D-rhamnoside, 4-nitrophenyl alpha-D-sialoside, and 4-nitrophenyl beta-D-GlcUA Saccharomyces cerevisiae BY4741 ?
-
 ?
sitosteryl beta-D-glucoside + H2O
-
 Saccharomyces cerevisiae sitosterol + beta-D-glucose
-
 ?

Subunits

Subunits Comment Organism
? x * 138000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
Egh1
-
 Saccharomyces cerevisiae
ergosteryl-beta-glucosidase
-
 Saccharomyces cerevisiae
Yir007w
-
 Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
4.5 6.5 about 90% activity at pH 4.5, 100% activity at pH 5.0, about 70% activity at pH 5.5, about 60% activity at pH 6.0, about 50% activity at pH 6.5, about 20% activity at pH 7.0, no activity at pH 8.5 Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction enzyme gene disruption results in the accumulation of ergosteryl beta-D-glucoside and fragmentation of vacuoles Saccharomyces cerevisiae
physiological function the enzyme is involved in sterylglucoside catabolism and vacuole formation in Saccharomyces cerevisiae Saccharomyces cerevisiae