BRENDA - Reference to 3.2.1.62; Id = 732073

EDTA

about 120% activity at 1 mM

Cryptococcus neoformans

expressed in Escherichia coli BL21(DE3) cells

Cryptococcus neoformans

E254Q

inactive

Cryptococcus neoformans

E483Q

inactive

Cryptococcus neoformans

Ca2+

about 78% residual activity at 1 mM

Cryptococcus neoformans

Co2+

about 18% residual activity at 1 mM

Cryptococcus neoformans

Cu2+

about 10% residual activity at 1 mM

Cryptococcus neoformans

DMSO

the enzyme is inhibited by 30-50% (v/v) DMSO

Cryptococcus neoformans

Fe2+

about 40% residual activity at 1 mM

Cryptococcus neoformans

Fe3+

about 50% residual activity at 1 mM

Cryptococcus neoformans

Hg+

complete inhibition at 1 mM

Cryptococcus neoformans

Mg2+

about 90% residual activity at 1 mM

Cryptococcus neoformans

Mn2+

about 50% residual activity at 1 mM

Cryptococcus neoformans

Na+

about 93% residual activity at 1 mM

Cryptococcus neoformans

Ni2+

about 5% residual activity at 1 mM

Cryptococcus neoformans

Pb2+

about 10% residual activity at 1 mM

Cryptococcus neoformans

Triton X-100

strongly inhibits the activity even at a low concentration

Cryptococcus neoformans

Zn2+

about 8% residual activity at 1 mM

Cryptococcus neoformans

0.0058

-

C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide

at pH 7.3 and 30°C

Cryptococcus neoformans

membrane

-

Cryptococcus neoformans

16020

-

additional information

no metal ions are required for the activity of isoform EGCrP1

Cryptococcus neoformans

oligoglycosylglucosyl-(1->1)-ceramide + H2O

Cryptococcus neoformans

-

ceramide + oligoglycosylglucose

-

?

oligoglycosylglucosyl-(1->1)-ceramide + H2O

Cryptococcus neoformans H99

-

ceramide + oligoglycosylglucose

-

?

DMSO

the enzyme is stimulated by 10-20% (v/v) DMSO

Cryptococcus neoformans

H1AE12

-

Cryptococcus neoformans H99

H1AE12

-

GSTrap column chromatography

Cryptococcus neoformans

C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide + H2O

highest activity

732073

Cryptococcus neoformans

?

-

?

C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide + H2O

highest activity

732073

Cryptococcus neoformans H99

?

-

?

glucosylceramide + H2O

low activity

732073

Cryptococcus neoformans

?

-

?

glucosylceramide + H2O

low activity

732073

Cryptococcus neoformans H99

?

-

?

oligoglycosylglucosyl-(1->1)-ceramide + H2O

-

732073

Cryptococcus neoformans

ceramide + oligoglycosylglucose

-

?

oligoglycosylglucosyl-(1->1)-ceramide + H2O

-

732073

Cryptococcus neoformans H99

ceramide + oligoglycosylglucose

-

?

EGCrP1

-

Cryptococcus neoformans

endoglycoceramidase-related protein 1

-

Cryptococcus neoformans

glucocerebrosidase

-

Cryptococcus neoformans

30

35

-

Cryptococcus neoformans

15

40

about 50% activity at 15°C, about 65% activity at 20°C, 100% activity at 30-35°C, about 30% activity at 40°C

Cryptococcus neoformans

0.0383

-

C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide

at pH 7.3 and 30°C

Cryptococcus neoformans

7.5

-

Cryptococcus neoformans

EDTA

about 120% activity at 1 mM

Cryptococcus neoformans

expressed in Escherichia coli BL21(DE3) cells

Cryptococcus neoformans

E254Q

inactive

Cryptococcus neoformans

E483Q

inactive

Cryptococcus neoformans

Ca2+

about 78% residual activity at 1 mM

Cryptococcus neoformans

Co2+

about 18% residual activity at 1 mM

Cryptococcus neoformans

Cu2+

about 10% residual activity at 1 mM

Cryptococcus neoformans

DMSO

the enzyme is inhibited by 30-50% (v/v) DMSO

Cryptococcus neoformans

Fe2+

about 40% residual activity at 1 mM

Cryptococcus neoformans

Fe3+

about 50% residual activity at 1 mM

Cryptococcus neoformans

Hg+

complete inhibition at 1 mM

Cryptococcus neoformans

Mg2+

about 90% residual activity at 1 mM

Cryptococcus neoformans

Mn2+

about 50% residual activity at 1 mM

Cryptococcus neoformans

Na+

about 93% residual activity at 1 mM

Cryptococcus neoformans

Ni2+

about 5% residual activity at 1 mM

Cryptococcus neoformans

Pb2+

about 10% residual activity at 1 mM

Cryptococcus neoformans

Triton X-100

strongly inhibits the activity even at a low concentration

Cryptococcus neoformans

Zn2+

about 8% residual activity at 1 mM

Cryptococcus neoformans

0.0058

-

C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide

at pH 7.3 and 30°C

Cryptococcus neoformans

membrane

-

Cryptococcus neoformans

16020

-

additional information

no metal ions are required for the activity of isoform EGCrP1

Cryptococcus neoformans

oligoglycosylglucosyl-(1->1)-ceramide + H2O

Cryptococcus neoformans

-

ceramide + oligoglycosylglucose

-

?

oligoglycosylglucosyl-(1->1)-ceramide + H2O

Cryptococcus neoformans H99

-

ceramide + oligoglycosylglucose

-

?

DMSO

the enzyme is stimulated by 10-20% (v/v) DMSO

Cryptococcus neoformans

GSTrap column chromatography

Cryptococcus neoformans

C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide + H2O

highest activity

732073

Cryptococcus neoformans

?

-

?

C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide + H2O

highest activity

732073

Cryptococcus neoformans H99

?

-

?

glucosylceramide + H2O

low activity

732073

Cryptococcus neoformans

?

-

?

glucosylceramide + H2O

low activity

732073

Cryptococcus neoformans H99

?

-

?

oligoglycosylglucosyl-(1->1)-ceramide + H2O

-

732073

Cryptococcus neoformans

ceramide + oligoglycosylglucose

-

?

oligoglycosylglucosyl-(1->1)-ceramide + H2O

-

732073

Cryptococcus neoformans H99

ceramide + oligoglycosylglucose

-

?

30

35

-

Cryptococcus neoformans

15

40

about 50% activity at 15°C, about 65% activity at 20°C, 100% activity at 30-35°C, about 30% activity at 40°C

Cryptococcus neoformans

0.0383

-

C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide

at pH 7.3 and 30°C

Cryptococcus neoformans

7.5

-

Cryptococcus neoformans

malfunction

gene disruption results in the accumulation of fungus-specific glucosylceramide and immature glucosylceramide that possess sphingoid bases without a methyl substitution concomitant with a dysfunction of polysaccharide capsule formation

Cryptococcus neoformans

metabolism

the enzyme participates in the catabolism of glucosylceramide and especially functions to eliminate immature glucosylceramide in vivo that are generated as by-products due to the broad specificity of glucosylceramide synthase

Cryptococcus neoformans

malfunction

gene disruption results in the accumulation of fungus-specific glucosylceramide and immature glucosylceramide that possess sphingoid bases without a methyl substitution concomitant with a dysfunction of polysaccharide capsule formation

Cryptococcus neoformans

metabolism

the enzyme participates in the catabolism of glucosylceramide and especially functions to eliminate immature glucosylceramide in vivo that are generated as by-products due to the broad specificity of glucosylceramide synthase

Cryptococcus neoformans

6.6

-

C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide

at pH 7.3 and 30°C

Cryptococcus neoformans

6.6

-

C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide

at pH 7.3 and 30°C

Cryptococcus neoformans

749451

Durand

-

A single point mutation alter ...

Rhodococcus sp. M-777

ACS Catal.

6

8264-8275

2016

-

1

-

1

-

3

-

1

-

1

-

5

-

2

-

3

-

1

-

1

-

3

-

1

-

5

-

3

-

3

749905

Ben Bdira

Hydrophobic interactions cont ...

Rhodococcus sp. M777

Biochemistry

55

4823-4835

2016

-

1

-

1

-

1

-

2

-

1

-

2

1

2

1

-

2

-

1

-

1

-

1

-

1

-

1

-

1

-

2

1

-

2

-

1

-

750706

Watanabe

Ergosteryl-beta-glucosidase ( ...

Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4741

Glycobiology

25

1079-1089

2015

-

1

-

69

-

1

-

20

1

3

-

1

-

1

-

1

-

20

1

-

1

-

2

-

732073

Ishibashi

Quality control of fungus-spec ...

Cryptococcus neoformans, Cryptococcus neoformans H99

J. Biol. Chem.

287

368-381

2012

1

-

1

-

2

-

14

1

-

2

1

5

-

1

-

6

-

3

1

-

1

-

1

-

1

-

2

-

14

-

1

-

2

1

-

1

-

6

-

1

-

1

-

2

-

1

715230

Naz

Epigallocatechin-3-gallate inh ...

Rattus norvegicus

J. Agric. Food Chem.

59

2734-2738

2011

-

1

-

1

-

1

-

1

-

2

-

1

-

1

-

1

-

1

-

709142

Behrendt

Structural hierarchy of regula ...

Homo sapiens

J. Biol. Chem.

285

4143-4152

2010

-

1

-

1

-

1

-

3

-

1

-

1

2

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

709206

Koek

The T-13910C polymorphism in t ...

Homo sapiens

J. Bone Miner. Res.

25

1980-1987

2010

-

1

-

1

-

1

-

2

-

1

-

1

-

698078

Behrendt

Impaired trafficking and subce ...

Homo sapiens

Gastroenterology

136

2295-2303

2009

-

1

-

2

-

1

-

2

-

2

-

1

-

2

-

2

-

698508

Willing

Intestinal microbiota differen ...

Sus scrofa

J. Anim. Physiol. Anim. Nutr. (Berl.)

93

586-595

2008

-

1

-

1

-

1

-

2

-

1

-

1

-

699936

Tanaka

Higher expression of jejunal L ...

Rattus norvegicus

Life Sci.

83

122-127

2008

1

-

1

-

1

-

1

-

2

-

3

-

2

-

1

-

1

-

1

-

1

-

3

-

701338

Cramm-Behrens

Apical cargo traverses endosom ...

Homo sapiens

Traffic

9

2206-2220

2008

1

-

1

-

1

-

2

-

2

-

1

-

1

-

1

-

677473

Bosse

Gata4 and Hnf1alpha are partia ...

Mus musculus

Am. J. Physiol. Gastrointest. Liver Physiol.

292

G1302-G1314

2007

-

1

-

3

-

2

-

1

-

4

-

1

-

2

-

1

-

679123

Beau

An NSP4-dependant mechanism by ...

Homo sapiens

Cell. Microbiol.

9

2254-2266

2007

-

1

-

1

2

-

2

-

4

-

1

-

1

6

-

2

-

4

-

1

-

1

-

2

-

2

-

1

-

1

6

-

2

-

680770

Caines

Structural and mechanistic ana ...

Rhodococcus sp.

J. Biol. Chem.

282

14300-14308

2007

-

1

2

-

2

-

2

-

1

-

2

-

3

-

1

-

1

2

-

2

-

1

-

2

-

680835

Hayashi

Klotho-related protein is a no ...

Danio rerio, Homo sapiens, Mus musculus, Rattus norvegicus, Tetraodontidae

J. Biol. Chem.

282

30889-30900

2007

1

-

2

1

4

-

1

6

8

-

1

-

9

-

1

-

13

10

-

18

-

19

-

1

-

1

-

2

-

1

4

-

1

-

6

8

-

1

-

1

-

13

10

-

18

-

1

-

666149

Mizuma

Kinetic characterization of gl ...

Homo sapiens

J. Pharm. Pharmacol.

57

661-664

2005

-

6

-

1

-

1

-

3

-

2

-

6

-

1

-

3

-

654552

Day

Absorption of quercetin-3-gluc ...

Rattus norvegicus

Biochem. Pharmacol.

65

1199-1206

2003

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

656600

Sesink

Intestinal uptake of quercetin ...

Rattus norvegicus

J. Nutr.

133

773-776

2003

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

657409

Wilkinson

Hydrolysis by lactase phlorizi ...

Rattus norvegicus

Xenobiotica

33

255-264

2003

-

1

-

1

-

1

-

1

-

1

-

2

-

1

-

1

-

1

-

1

-

2

-

656061

Mackey

Enzymatic hydrolysis of pyrido ...

Rattus norvegicus

J. Biol. Chem.

277

26858-26864

2002

-

5

2

1

-

2

-

1

-

1

-

2

1

-

1

-

2

-

5

2

1

-

1

-

1

-

2

1

-

1

-

656110

Jacob

Prosequence of human lactase-p ...

Homo sapiens

J. Biol. Chem.

277

8217-8225

2002

-

2

-

2

-

2

-

656599

Burrin

Oral IGF-I alters the posttran ...

Sus scrofa

J. Nutr.

131

2235-2241

2001

-

2

-

1

-

2

-

1

-

2

-

208743

Day

Dietary flavonoids and isoflav ...

Ovis aries, Ovis aries aries

FEBS Lett.

468

166-170

2000

-

2

12

1

-

3

-

3

-

1

-

2

-

14

-

12

-

2

-

12

1

-

3

-

1

-

2

-

14

-

12

-

208744

Jacob

Additional N-glycosylation and ...

Homo sapiens

J. Biol. Chem.

275

10630-10637

2000

-

1

-

3

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

3

-

1

-

1

-

1

-

1

-

1

-

655412

Arribas

Differential mechanism-based l ...

Ovis aries

Eur. J. Biochem.

267

6996-7005

2000

-

4

-

2

-

1

-

4

-

1

-

208745

Wuthrich

Human lactase-phloizin hydrola ...

Homo sapiens

FEBS Lett.

405

321-327

1997

-

1

-

3

-

2

1

-

2

-

1

-

4

-

1

-

1

-

3

-

2

1

-

1

-

4

-

1

-

208746

Estrada

Quantitative analysis of lacta ...

Rattus sp.

J. Cell. Physiol.

167

341-348

1996

-

1

-

2

-

4

-

1

-

1

-

4

-

1

-

208747

Naim

The pro-region of human intest ...

Homo sapiens

Biol. Chem. Hoppe-Seyler

376

255-258

1995

-

1

-

2

1

-

2

-

1

-

1

-

1

-

1

-

2

1

-

1

-

1

-

1

-

208748

Naim

Processing and transport of hu ...

Homo sapiens

FEBS Lett.

342

302-307

1994

-

1

-

1

-

2

-

1

-

3

-

1

-

1

-

1

-

1

-

1

-

3

-

1

-

208749

Naim

Impact of O-glycosylation on t ...

Homo sapiens

J. Biol. Chem.

267

25494-25504

1992

-

1

-

2

1

-

3

-

1

-

2

-

1

-

1

-

1

-

2

1

-

1

-

2

-

1

-

208750

Wacker

Location of the two catalytic ...

Oryctolagus cuniculus

J. Biol. Chem.

267

18744-18752

1992

-

3

-

3

-

1

3

-

2

-

1

-

1

-

4

1

-

2

-

3

2

-

3

-

1

3

-

1

-

1

-

4

1

-

208751

Mantei

Complete primary structure of ...

Homo sapiens, Oryctolagus cuniculus

EMBO J.

7

2705-2713

1988

-

4

-

2

-

4

-

1

-

2

-

3

1

-

4

-

2

-

1

-

2

-

3

1

-

208752

Buller

Biosynthesis, glycosylation, a ...

Rattus sp.

J. Biol. Chem.

262

17206-17211

1987

-

3

-

2

-

3

-

1

-

3

-

1

-

3

-

2

-

1

-

3

-

1

-

208753

Naim

Biosynthesis and maturation of ...

Homo sapiens

Biochem. J.

241

427-434

1987

-

2

-

2

-

1

-

7

-

1

-

2

-

1

-

7

-

1

-

208754

Danielsen

Biosynthesis of intestinal mic ...

Sus scrofa

Biochem. Biophys. Res. Commun.

122

82-90

1984

-

1

-

2

-

2

-

1

-

3

-

1

-

1

-

2

-

1

-

3

-

1

-

208755

Skovbjerg

Evidence for biosynthesis of l ...

Homo sapiens

Biochim. Biophys. Acta

798

247-251

1984

-

2

-

2

-

2

-

1

-

2

-

2

-

1

-

208757

Skovbjerg

Further characterization of in ...

Homo sapiens, Sus scrofa

Biochim. Biophys. Acta

707

89-97

1982

-

4

1

2

-

3

16

-

2

-

1

-

2

-

26

2

-

1

-

1

-

1

-

4

-

1

2

-

3

16

-

1

-

2

-

26

2

-

1

-

1

-

1

-

208756

Skovbjerg

Purification and characterizat ...

Homo sapiens

Eur. J. Biochem.

114

653-661

1981

-

1

2

3

1

-

3

4

-

1

-

1

-

1

7

1

-

1

-

1

-

1

-

1

-

2

-

3

1

-

3

4

-

1

-

1

7

1

-

1

-

1

-

1

-

208758

Kobayashi

The glycosylceramidase in the ...

Mus musculus

J. Biol. Chem.

256

7768-7773

1981

1

-

17

-

1

12

-

4

-

1

-

3

-

18

-

1

-

2

-

1

-

17

-

1

12

-

1

-

3

-

18

-

1

-

2

-

208759

Cousineau

Isolation and characterization ...

Rattus sp.

Biochim. Biophys. Acta

615

147-157

1980

-

4

1

-

2

-

2

-

1

-

2

1

-

2

-

1

-

1

-

4

1

-

2

-

1

-

2

1

-

2

-

1

-

1

-

208760

Ramaswamy

Lactase-phlorizin hydrolase co ...

Macaca radiata

Biochim. Biophys. Acta

403

446-455

1975

-

3

2

-

6

-

2

-

1

-

2

-

12

-

1

-

1

-

1

-

3

1

2

-

6

-

1

-

2

-

12

-

1

-

1

-

208761

Birkenmeier

Enzymatic properties of rat la ...

Rattus sp.

Biochim. Biophys. Acta

350

100-112

1974

-

1

-

1

-

5

-

2

-

1

-

1

-

1

8

-

1

-

1

-

5

-

1

-

1

-

1

8

-

208762

Leese

On the identity between the sm ...

Rattus sp.

J. Biol. Chem.

248

8170-8173

1973

-

4

2

-

4

-

1

-

1

-

1

-

15

-

1

-

1

-

4

-

2

-

4

-

1

-

1

-

15

-

1

-

1

-

208763

Hamaswamy

Lactase-phlorizin hydrolase co ...

Macaca radiata

Biochem. Biophys. Res. Commun.

54

197-204

1973

6

-

7

-

2

-

2

-

1

-

2

1

-

2

-

2

-

6

-

7

-

2

-

1

-

2

1

-

2

-

2

-

208764

Kraml

beta-Glucosidase (phlorizin hy ...

Rattus sp.

Biochim. Biophys. Acta

258

520-530

1972

-

4

7

1

-

3

-

1

-

1

-

1

-

7

-

4

-

7

1

-

3

-

1

-

1

-

7

-

208765

Malathi

Phlorizin hydrolase: A beta-gl ...

Cricetinae

Biochim. Biophys. Acta

173

245-256

1969

-

2

1

2

-

1

-

1

-

1

-

1

-

1

-

2

-

1

2

-

1

-

1

-

1

-

1

-

Quality control of fungus-specific glucosylceramide in Cryptococcus neoformans by endoglycoceramidase-related protein 1 (EGCrP1)

Data extracted from this reference: