Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | about 120% activity at 1 mM | Cryptococcus neoformans |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Cryptococcus neoformans |
Protein Variants | Comment | Organism |
---|---|---|
E254Q | inactive | Cryptococcus neoformans |
E483Q | inactive | Cryptococcus neoformans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | about 78% residual activity at 1 mM | Cryptococcus neoformans | |
Co2+ | about 18% residual activity at 1 mM | Cryptococcus neoformans | |
Cu2+ | about 10% residual activity at 1 mM | Cryptococcus neoformans | |
DMSO | the enzyme is inhibited by 30-50% (v/v) DMSO | Cryptococcus neoformans | |
Fe2+ | about 40% residual activity at 1 mM | Cryptococcus neoformans | |
Fe3+ | about 50% residual activity at 1 mM | Cryptococcus neoformans | |
Hg+ | complete inhibition at 1 mM | Cryptococcus neoformans | |
Mg2+ | about 90% residual activity at 1 mM | Cryptococcus neoformans | |
Mn2+ | about 50% residual activity at 1 mM | Cryptococcus neoformans | |
Na+ | about 93% residual activity at 1 mM | Cryptococcus neoformans | |
Ni2+ | about 5% residual activity at 1 mM | Cryptococcus neoformans | |
Pb2+ | about 10% residual activity at 1 mM | Cryptococcus neoformans | |
Triton X-100 | strongly inhibits the activity even at a low concentration | Cryptococcus neoformans | |
Zn2+ | about 8% residual activity at 1 mM | Cryptococcus neoformans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0058 | - |
C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide | at pH 7.3 and 30°C | Cryptococcus neoformans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Cryptococcus neoformans | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no metal ions are required for the activity of isoform EGCrP1 | Cryptococcus neoformans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
oligoglycosylglucosyl-(1->1)-ceramide + H2O | Cryptococcus neoformans | - |
ceramide + oligoglycosylglucose | - |
? | |
oligoglycosylglucosyl-(1->1)-ceramide + H2O | Cryptococcus neoformans H99 | - |
ceramide + oligoglycosylglucose | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
DMSO | the enzyme is stimulated by 10-20% (v/v) DMSO | Cryptococcus neoformans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cryptococcus neoformans | H1AE12 | - |
- |
Cryptococcus neoformans H99 | H1AE12 | - |
- |
Purification (Comment) | Organism |
---|---|
GSTrap column chromatography | Cryptococcus neoformans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide + H2O | highest activity | Cryptococcus neoformans | ? | - |
? | |
C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide + H2O | highest activity | Cryptococcus neoformans H99 | ? | - |
? | |
glucosylceramide + H2O | low activity | Cryptococcus neoformans | ? | - |
? | |
glucosylceramide + H2O | low activity | Cryptococcus neoformans H99 | ? | - |
? | |
oligoglycosylglucosyl-(1->1)-ceramide + H2O | - |
Cryptococcus neoformans | ceramide + oligoglycosylglucose | - |
? | |
oligoglycosylglucosyl-(1->1)-ceramide + H2O | - |
Cryptococcus neoformans H99 | ceramide + oligoglycosylglucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EGCrP1 | - |
Cryptococcus neoformans |
endoglycoceramidase-related protein 1 | - |
Cryptococcus neoformans |
glucocerebrosidase | - |
Cryptococcus neoformans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 35 | - |
Cryptococcus neoformans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 40 | about 50% activity at 15°C, about 65% activity at 20°C, 100% activity at 30-35°C, about 30% activity at 40°C | Cryptococcus neoformans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0383 | - |
C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide | at pH 7.3 and 30°C | Cryptococcus neoformans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Cryptococcus neoformans |
General Information | Comment | Organism |
---|---|---|
malfunction | gene disruption results in the accumulation of fungus-specific glucosylceramide and immature glucosylceramide that possess sphingoid bases without a methyl substitution concomitant with a dysfunction of polysaccharide capsule formation | Cryptococcus neoformans |
metabolism | the enzyme participates in the catabolism of glucosylceramide and especially functions to eliminate immature glucosylceramide in vivo that are generated as by-products due to the broad specificity of glucosylceramide synthase | Cryptococcus neoformans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.6 | - |
C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide | at pH 7.3 and 30°C | Cryptococcus neoformans |