BRENDA - Enzyme Database
show all sequences of 3.2.1.62

Quality control of fungus-specific glucosylceramide in Cryptococcus neoformans by endoglycoceramidase-related protein 1 (EGCrP1)

Ishibashi, Y.; Ikeda, K.; Sakaguchi, K.; Okino, N.; Taguchi, R.; Ito, M.; J. Biol. Chem. 287, 368-381 (2012)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
EDTA
about 120% activity at 1 mM
Cryptococcus neoformans
Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Cryptococcus neoformans
Engineering
Amino acid exchange
Commentary
Organism
E254Q
inactive
Cryptococcus neoformans
E483Q
inactive
Cryptococcus neoformans
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
about 78% residual activity at 1 mM
Cryptococcus neoformans
Co2+
about 18% residual activity at 1 mM
Cryptococcus neoformans
Cu2+
about 10% residual activity at 1 mM
Cryptococcus neoformans
DMSO
the enzyme is inhibited by 30-50% (v/v) DMSO
Cryptococcus neoformans
Fe2+
about 40% residual activity at 1 mM
Cryptococcus neoformans
Fe3+
about 50% residual activity at 1 mM
Cryptococcus neoformans
Hg+
complete inhibition at 1 mM
Cryptococcus neoformans
Mg2+
about 90% residual activity at 1 mM
Cryptococcus neoformans
Mn2+
about 50% residual activity at 1 mM
Cryptococcus neoformans
Na+
about 93% residual activity at 1 mM
Cryptococcus neoformans
Ni2+
about 5% residual activity at 1 mM
Cryptococcus neoformans
Pb2+
about 10% residual activity at 1 mM
Cryptococcus neoformans
Triton X-100
strongly inhibits the activity even at a low concentration
Cryptococcus neoformans
Zn2+
about 8% residual activity at 1 mM
Cryptococcus neoformans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0058
-
C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide
at pH 7.3 and 30°C
Cryptococcus neoformans
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Cryptococcus neoformans
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no metal ions are required for the activity of isoform EGCrP1
Cryptococcus neoformans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
oligoglycosylglucosyl-(1->1)-ceramide + H2O
Cryptococcus neoformans
-
ceramide + oligoglycosylglucose
-
-
?
oligoglycosylglucosyl-(1->1)-ceramide + H2O
Cryptococcus neoformans H99
-
ceramide + oligoglycosylglucose
-
-
?
Organic Solvent Stability
Organic Solvent
Commentary
Organism
DMSO
the enzyme is stimulated by 10-20% (v/v) DMSO
Cryptococcus neoformans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cryptococcus neoformans
H1AE12
-
-
Cryptococcus neoformans H99
H1AE12
-
-
Purification (Commentary)
Commentary
Organism
GSTrap column chromatography
Cryptococcus neoformans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide + H2O
highest activity
732073
Cryptococcus neoformans
?
-
-
-
?
C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide + H2O
highest activity
732073
Cryptococcus neoformans H99
?
-
-
-
?
glucosylceramide + H2O
low activity
732073
Cryptococcus neoformans
?
-
-
-
?
glucosylceramide + H2O
low activity
732073
Cryptococcus neoformans H99
?
-
-
-
?
oligoglycosylglucosyl-(1->1)-ceramide + H2O
-
732073
Cryptococcus neoformans
ceramide + oligoglycosylglucose
-
-
-
?
oligoglycosylglucosyl-(1->1)-ceramide + H2O
-
732073
Cryptococcus neoformans H99
ceramide + oligoglycosylglucose
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
35
-
Cryptococcus neoformans
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
15
40
about 50% activity at 15°C, about 65% activity at 20°C, 100% activity at 30-35°C, about 30% activity at 40°C
Cryptococcus neoformans
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0383
-
C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide
at pH 7.3 and 30°C
Cryptococcus neoformans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Cryptococcus neoformans
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
EDTA
about 120% activity at 1 mM
Cryptococcus neoformans
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Cryptococcus neoformans
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
E254Q
inactive
Cryptococcus neoformans
E483Q
inactive
Cryptococcus neoformans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
about 78% residual activity at 1 mM
Cryptococcus neoformans
Co2+
about 18% residual activity at 1 mM
Cryptococcus neoformans
Cu2+
about 10% residual activity at 1 mM
Cryptococcus neoformans
DMSO
the enzyme is inhibited by 30-50% (v/v) DMSO
Cryptococcus neoformans
Fe2+
about 40% residual activity at 1 mM
Cryptococcus neoformans
Fe3+
about 50% residual activity at 1 mM
Cryptococcus neoformans
Hg+
complete inhibition at 1 mM
Cryptococcus neoformans
Mg2+
about 90% residual activity at 1 mM
Cryptococcus neoformans
Mn2+
about 50% residual activity at 1 mM
Cryptococcus neoformans
Na+
about 93% residual activity at 1 mM
Cryptococcus neoformans
Ni2+
about 5% residual activity at 1 mM
Cryptococcus neoformans
Pb2+
about 10% residual activity at 1 mM
Cryptococcus neoformans
Triton X-100
strongly inhibits the activity even at a low concentration
Cryptococcus neoformans
Zn2+
about 8% residual activity at 1 mM
Cryptococcus neoformans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0058
-
C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide
at pH 7.3 and 30°C
Cryptococcus neoformans
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Cryptococcus neoformans
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no metal ions are required for the activity of isoform EGCrP1
Cryptococcus neoformans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
oligoglycosylglucosyl-(1->1)-ceramide + H2O
Cryptococcus neoformans
-
ceramide + oligoglycosylglucose
-
-
?
oligoglycosylglucosyl-(1->1)-ceramide + H2O
Cryptococcus neoformans H99
-
ceramide + oligoglycosylglucose
-
-
?
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
DMSO
the enzyme is stimulated by 10-20% (v/v) DMSO
Cryptococcus neoformans
Purification (Commentary) (protein specific)
Commentary
Organism
GSTrap column chromatography
Cryptococcus neoformans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide + H2O
highest activity
732073
Cryptococcus neoformans
?
-
-
-
?
C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide + H2O
highest activity
732073
Cryptococcus neoformans H99
?
-
-
-
?
glucosylceramide + H2O
low activity
732073
Cryptococcus neoformans
?
-
-
-
?
glucosylceramide + H2O
low activity
732073
Cryptococcus neoformans H99
?
-
-
-
?
oligoglycosylglucosyl-(1->1)-ceramide + H2O
-
732073
Cryptococcus neoformans
ceramide + oligoglycosylglucose
-
-
-
?
oligoglycosylglucosyl-(1->1)-ceramide + H2O
-
732073
Cryptococcus neoformans H99
ceramide + oligoglycosylglucose
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
35
-
Cryptococcus neoformans
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
15
40
about 50% activity at 15°C, about 65% activity at 20°C, 100% activity at 30-35°C, about 30% activity at 40°C
Cryptococcus neoformans
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0383
-
C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide
at pH 7.3 and 30°C
Cryptococcus neoformans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Cryptococcus neoformans
General Information
General Information
Commentary
Organism
malfunction
gene disruption results in the accumulation of fungus-specific glucosylceramide and immature glucosylceramide that possess sphingoid bases without a methyl substitution concomitant with a dysfunction of polysaccharide capsule formation
Cryptococcus neoformans
metabolism
the enzyme participates in the catabolism of glucosylceramide and especially functions to eliminate immature glucosylceramide in vivo that are generated as by-products due to the broad specificity of glucosylceramide synthase
Cryptococcus neoformans
General Information (protein specific)
General Information
Commentary
Organism
malfunction
gene disruption results in the accumulation of fungus-specific glucosylceramide and immature glucosylceramide that possess sphingoid bases without a methyl substitution concomitant with a dysfunction of polysaccharide capsule formation
Cryptococcus neoformans
metabolism
the enzyme participates in the catabolism of glucosylceramide and especially functions to eliminate immature glucosylceramide in vivo that are generated as by-products due to the broad specificity of glucosylceramide synthase
Cryptococcus neoformans
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
6.6
-
C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide
at pH 7.3 and 30°C
Cryptococcus neoformans
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
6.6
-
C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide
at pH 7.3 and 30°C
Cryptococcus neoformans
Other publictions for EC 3.2.1.62
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749451
Durand
-
A single point mutation alter ...
Rhodococcus sp. M-777
ACS Catal.
6
8264-8275
2016
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1
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1
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3
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5
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3
-
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3
3
749905
Ben Bdira
Hydrophobic interactions cont ...
Rhodococcus sp. M777
Biochemistry
55
4823-4835
2016
-
-
1
1
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1
-
-
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1
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2
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1
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2
1
1
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2
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1
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1
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1
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1
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1
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2
1
1
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2
-
1
-
-
-
-
-
-
-
-
-
750706
Watanabe
Ergosteryl-beta-glucosidase ( ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4741
Glycobiology
25
1079-1089
2015
-
-
1
-
-
-
-
-
-
-
-
-
-
62
-
-
1
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20
1
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1
1
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1
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1
-
-
-
-
20
1
-
-
-
-
1
1
-
-
-
2
2
-
-
-
732073
Ishibashi
Quality control of fungus-spec ...
Cryptococcus neoformans, Cryptococcus neoformans H99
J. Biol. Chem.
287
368-381
2012
1
-
1
-
2
-
14
1
1
1
-
2
1
5
-
-
1
-
-
-
-
-
6
-
1
1
-
1
1
-
-
-
-
-
-
1
-
1
-
-
2
-
-
14
-
1
1
1
-
2
1
-
-
1
-
-
-
-
6
-
1
1
-
1
1
-
-
-
-
2
2
-
1
1
715230
Naz
Epigallocatechin-3-gallate inh ...
Rattus norvegicus
J. Agric. Food Chem.
59
2734-2738
2011
-
-
-
-
-
-
1
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-
-
-
-
1
-
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1
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1
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1
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1
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1
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-
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-
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-
-
-
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-
709142
Behrendt
Structural hierarchy of regula ...
Homo sapiens
J. Biol. Chem.
285
4143-4152
2010
-
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1
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1
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1
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3
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1
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1
1
1
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1
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1
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1
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1
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1
-
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1
1
1
-
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-
1
-
-
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-
1
1
-
-
-
709206
Koek
The T-13910C polymorphism in t ...
Homo sapiens
J. Bone Miner. Res.
25
1980-1987
2010
-
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1
-
1
-
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-
-
-
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1
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-
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698078
Behrendt
Impaired trafficking and subce ...
Homo sapiens
Gastroenterology
136
2295-2303
2009
-
1
1
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2
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1
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1
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2
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2
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698508
Willing
Intestinal microbiota differen ...
Sus scrofa
J. Anim. Physiol. Anim. Nutr. (Berl.)
93
586-595
2008
-
-
-
-
-
-
1
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1
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-
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-
-
-
-
-
-
-
-
-
-
-
-
699936
Tanaka
Higher expression of jejunal L ...
Rattus norvegicus
Life Sci.
83
122-127
2008
1
-
1
-
-
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1
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1
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2
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3
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1
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1
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1
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1
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3
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-
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-
701338
Cramm-Behrens
Apical cargo traverses endosom ...
Homo sapiens
Traffic
9
2206-2220
2008
1
1
1
-
-
-
1
-
1
-
-
-
-
2
-
-
-
-
-
-
-
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1
1
1
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1
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-
-
677473
Bosse
Gata4 and Hnf1alpha are partia ...
Mus musculus
Am. J. Physiol. Gastrointest. Liver Physiol.
292
G1302-G1314
2007
-
1
-
-
-
-
-
-
-
-
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3
-
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2
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1
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1
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2
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1
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-
679123
Beau
An NSP4-dependant mechanism by ...
Homo sapiens
Cell. Microbiol.
9
2254-2266
2007
-
1
-
-
-
-
1
2
-
-
2
-
-
4
-
-
1
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1
6
-
2
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1
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1
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2
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2
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1
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1
6
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
680770
Caines
Structural and mechanistic ana ...
Rhodococcus sp.
J. Biol. Chem.
282
14300-14308
2007
-
-
1
1
2
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-
2
-
-
-
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2
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1
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7
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2
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1
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2
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2
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208764
Kraml
beta-Glucosidase (phlorizin hy ...
Rattus sp.
Biochim. Biophys. Acta
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520-530
1972
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4
7
1
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208765
Malathi
Phlorizin hydrolase: A beta-gl ...
Cricetinae
Biochim. Biophys. Acta
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245-256
1969
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