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Literature summary for 3.2.1.62 extracted from

  • Behrendt, M.; Polaina, J.; Naim, H.Y.
    Structural hierarchy of regulatory elements in the folding and transport of an intestinal multidomain protein (2010), J. Biol. Chem., 285, 4143-4152.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.62

Cloned(Commentary)

Cloned (Comment) Organism
transient expression of wild-type and domain deletion mutant enzymes and isolated enzyme domains in Cos-1 cells, expression in MDCK cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information loop-out mutagenesis and construction of deletion or individual domain forms of LPH. Removal of domain IV, which contains lactase, results in a diminished phlorizin hydrolase activity, lack of dimerization in the endoplasmic reticulum, but accelerated transport kinetics from the endoplasmic reticulum to the Golgi apparatus. By contrast, deletion of domain III, which harbors phlorizin hydrolase, generates a malfolded protein that is blocked in the endoplasmic reticulum. Transport kinetics and enzyme activity of LPH and deletion mutants differ Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phlorizin + H2O Homo sapiens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
intestine
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phlorizin + H2O
-
 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
More LPH encompasses four homologous domains, which presumably have evolved from two subsequent duplications of one ancestral gene, domain organization, overview. The profragment, LPHalpha, comprises homologous domains I and II and functions as an intramolecular chaperone in the context of the brush-border LPHbeta region of LPH. Inter-relationship between homologous domains III and IV of LPHbeta implication in the overall structure, function, and trafficking of LPH, overview. Isolated homologous domain III is transport-competent per se and sorted to the apical membrane in polarized cells. Nevertheless, it neither dimerizes nor acquires complete phlorizin hydrolase activity Homo sapiens

Synonyms

Synonyms Comment Organism
lactase-phlorizin hydrolase
-
 Homo sapiens
LPH
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

General Information

General Information Comment Organism
physiological function The profragment, LPHalpha, comprises homologous domains I and II and functions as an intramolecular chaperone in the context of the brush-border LPHbeta region of LPH Homo sapiens