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Literature summary for 3.2.1.62 extracted from

  • Kraml, J.; Kolinska, J.; Ellederova, D.; Hirsova, D.
    beta-Glucosidase (phlorizin hydrolase) activity of the lactase fraction isolated from the small intestinal mucosa of infant rats, and the relationship between beta-glucosidases and beta-galactosidases (1972), Biochim. Biophys. Acta, 258, 520-530.
    View publication on PubMed
Search for more literature for 3.2.1.62

Inhibitors

Inhibitors Comment Organism Structure
D-galactono-1,4-lactone competitive inhibition of lactase activity, Ki: 0.35 mM Rattus sp.
D-glucono-1,5-lactone competitive inhibition of lactase activity, Ki: 0.027 mM Rattus sp.
phlorizin competitive inhibition of lactase activity, Ki: 0.047 mM Rattus sp.
Tris(hydroxymethyl)aminomethane competitive inhibition of lactase activity, Ki: 4.5 mM Rattus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 phlorizin
-
 Rattus sp.
0.4
-
 phloretin-beta-D-galactoside
-
 Rattus sp.
1.1
-
 4-O-beta-D-glucopyranosyl-D-glucopyranoside
-
 Rattus sp.
4.1
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Rattus sp.
13.1
-
 2-nitrophenyl-beta-D-galactopyranoside
-
 Rattus sp.
22.3
-
 4-nitrophenyl-beta-D-galactopyranoside
-
 Rattus sp.
40
-
 4-O-beta-D-galactopyranosyl-D-glucopyranoside
-
 Rattus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
brush border
-
 Rattus sp. 5903
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O Rattus sp. best substrate D-galactose + beta-D-glucose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Rattus sp. cellobiose beta-D-glucose + D-glucose
-
 ?
phlorizin + H2O Rattus sp.
-
 phloretin + beta-D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
intestine
-
 Rattus sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Rattus sp. 2-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Rattus sp. 4-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Rattus sp. 4-nitrophenol + beta-D-glucose
-
 ?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O best substrate Rattus sp. D-galactose + beta-D-glucose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Rattus sp. beta-D-glucose + D-glucose
-
 ?
phloretin-beta-D-galactoside + H2O very low activity Rattus sp. phloretin + beta-D-galactose
-
 ?
phlorizin + H2O
-
 Rattus sp. phloretin + beta-D-glucose
-
 ?